کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8472108 | 1550292 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Erythroblast differentiation at spleen in Q137E mutant ribosomal protein S19 gene knock-in C57BL/6J mice
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کلمات کلیدی
FACSPHZDBACD54GPCRsCD11aCFU-EBFU-EC5aRERK1/2 - ERK1 / 2G protein-coupled receptors - G گیرنده های پروتئینی همراهembryonic stem - ساقه جنینlymphocyte function antigen-1 - عملکرد آنتیژن 1 لنفوسیتfluorescence activated cell sorting - فلورسانس سلول فعال فعال سلولPhenylhydrazine - فنیل هیدرازینintracellular adhesion molecule-1 - مولکول چسبندگی داخل سلولی -1Hemin - همینerythroid burst-forming units - واحدهای پاره شدن اریتروییدDiamond-Blackfan Anemia - کم خونی الماس سیاه فانextracellular signal-regulated kinase 1/2 - کیناز 1/2 تنظیم سیگنال خارج سلولیGlycophorin A - گلیکوفورین AC5a receptor - گیرنده C5a
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
We recently found that erythroblast-like cells derived from human leukaemia K562 cells express C5a receptor (C5aR) and produce its antagonistic and agonistic ligand ribosomal protein S19 (RP S19) polymer, which is cross-linked between K122 and Q137 by tissue transglutaminases. RP S19 polymer binds to the reciprocal C5aRs on erythroblast-like cells and macrophage-like cells derived from human monocytic THP-1 cells and promotes differentiation into reticulocyte-like cells through enucleation in vitro. To examine the roles of RP S19 polymer in mouse erythropoiesis, we prepared Q137E mutant RP S19 gene knock-in C57BL/6J mice. In contrast to wild-type mice, erythroblast numbers at the preliminary stage (CD71high/TER119low) in spleen based on transferrin receptor (CD71) and glycophorin A (TER119) values and erythrocyte numbers in orbital artery bloods were not largely changed in knock-in mice. Conversely, erythroblast numbers at the early stage (CD71high/TER119high) were significantly decreased in spleen by knock-in mice. The reduction of early erythroblast numbers in spleen was enhanced by the phenylhydrazine-induced pernicious anemia model knock-in mice and was rescued by a functional analogue of RP S19 dimer S-tagged C5a/RP S19. These data indicated that RP S19 polymer plays the roles in the early erythroblast differentiation of C57BL/6J mouse spleen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 223, Issue 1, January 2018, Pages 118-124
Journal: Immunobiology - Volume 223, Issue 1, January 2018, Pages 118-124
نویسندگان
Koji Yamanegi, Naoko Yamada, Keiji Nakasho, Hiroshi Nishiura,