کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8480978 | 1551408 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of 1,25 dihydroxyvitamin D3 on HCl/Ethanol-induced gastric injury in rats
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
This study evaluated ulceroprotective and antioxidant effect of 1,25 dihydroxyvitamin D3 against gastric damage in rats. Rats were treated intraperitoneally with either 1,25 dihydroxyvitamin D3 (0.25â¯Î¼g/kg) or saline for 14â¯days. On day-15, the non-selective cyclooxygenase inhibitor indomethacin (10â¯mg/kg; subcutaneously), the inhibitor of sulfhydryl groups N-ethylmaleimide (10â¯mg/kg; intraperitoneally) or ATP-sensitive K+ channel blocker glibenclamide (10â¯mg/kg; orally) was given prior to 1,25 dihydroxyvitamin D3. Animals were euthanized at 60â¯min post ulcerogenic challenge (0.3â¯M HCl and 60% ethanol (0.2â¯mL; orally). Stomach and blood were collected for biochemical and histological evaluations. HCl/Ethanol group revealed severely damaged mucous and glandular epithelium with diffuse hemorrhage and inflammatory cell infiltration (microscopic score: 10.67â¯Â±â¯0.67 and ulcer index: 33.13â¯Â±â¯5.09). 1,25 dihydroxyvitamin D3 decreased the extent of damage (microscopic score: 6.80â¯Â±â¯0.02 and ulcer index: 19.00â¯Â±â¯4.34; pâ¯<â¯0.05), and the elevations in gastric malondialdehyde level (pâ¯<â¯0.001), myeloperoxidase activity (pâ¯<â¯0.001), nuclear factor-κB expression (pâ¯<â¯0.05), and apoptotic index (pâ¯<â¯0.05) following HCl/Ethanol challenge. Decreased gastric glutathione following HCl/Ethanol administration was restored by 1,25 dihydroxyvitamin D3 (pâ¯<â¯0.01). These findings demonstrated protection of the gastric mucosa against HCl/Ethanol-induced injury by 1,25 dihydroxyvitamin D3 via attenuation of inflammatory reaction, oxidative stress and apoptosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tissue and Cell - Volume 51, April 2018, Pages 68-76
Journal: Tissue and Cell - Volume 51, April 2018, Pages 68-76
نویسندگان
Hasan Huseyin Sahin, Alev Cumbul, Unal Uslu, Zeynep Yilmaz, Feriha Ercan, Inci Alican,