Glycoconjugates pattern and chemosensory cells in the camel respiratory mucosa: Lectin and immunohistochemical studies

The glycoconjugates pattern of acidic secretions and distribution of chemosensory cells (SCCs) in the respiratory mucosa of dromedary camels were analyzed so as to identify their functional role. Secretions of the goblet cells and mucous glandular cells were analyzed to evaluate the variety of sugar chains, focusing on the acidic glycoconjugates. Using lectin histochemistry, WGA, STL, DBA, SBA, VVA and RCA-120 intensely bound to the goblet cells. PNA and ECL labeled the goblet cells with moderate intensity. While, s-WGA, UEA-I faintly bound to them. Lectins bound to the glycocalyx: WGA, LEL, STL, DSL, DBA, SBA, VVA, RCA-120, ECL and PHA-L (tetra- and tri-antennary N-glycans). The mucous secretory cells reacted with: WGA, s-WGA, STL, DBA, SBA, ECL and Con A. Glycoconjugates secreted by the camel respiratory mucosa are rich in sialomucins, glucosaminy-lated residuals with some galactosyl/galactosaminylated residues; few L-fucose and mannosylated sugar residues are also included. For identification of SCCs, the camel respiratory mucosa was immunostained with phospholipase C-β2 (PLC-β2), a taste signaling marker. Several PLC-β2 immunoreactive cells were detected in camel respiratory epithelium. Finally, prevalence of sialomucins and SCCs which can respond to noxious chemicals may suggest a vital role in optimizing physiological and pathological reactions in camel respiratory mucosa.