کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8482469 1551531 2018 41 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Seasonal and Dietary Influences on Adipose Tissue and Systemic Gene Expression in Control and Previously Laminitic Ponies
ترجمه فارسی عنوان
تأثیر فصلی و رژیم غذایی بر روی بافت چربی و بیان ژن سیستمیک در پانکودرهای کنترل و پیش از لامینی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
The aims of the study were to determine whether adipose tissue global gene expression (1) differs between never laminitic (NL) and previously laminitic (PL) ponies, (2) is influenced by season and/or a diet designed to simulate spring grass, and (3) differences seen also occur systemically in peripheral blood mononuclear cells (PBMCs). Subcutaneous adipose tissue and PBMCs were obtained from six NL and six PL ponies on three occasions; summer, winter (season study), and in winter after consuming a diet simulating spring grass for 7 days (diet study). Adipose tissue global gene expression was determined using a 44K equine-specific microarray, validated using multiplex quantitative real time polymerase chain reaction (qRT-PCR), and analyzed using GeneSpring software and Ingenuity Pathway Analysis. PBMC gene expression was quantified using qRT-PCR. The total number of genes whose expression differed (≥2-fold change, P ≤ .01) between PL and NL ponies was greater in summer (192 genes) compared to winter (58 genes); 40/192 genes influenced by disease in the summer were also seasonally regulated and were predominantly associated with inflammation. The genes modified by dietary intervention and PBMC gene expression did not follow the same pattern as the season study. Thus, adipose tissue global gene expression differed between NL and PL ponies most in summer compared to winter, and these differentially expressed genes predominantly related to inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Equine Veterinary Science - Volume 69, October 2018, Pages 84-95
نویسندگان
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