کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8483635 | 1551566 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Is Resveratrol Effective in Protecting Stallion Cooled Semen?
ترجمه فارسی عنوان
آیا رزوراترول در محافظت از سموم خنک کننده استالیون موثر است؟
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کلمات کلیدی
مایع منی، تبرید، رزوراترول،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
The aim of this work was to evaluate the effect of resveratrol (RSV) during liquid storage of stallion sperm for 24 hours at either 10°C or 4°C. The antioxidant RSV was added to reduce the oxidative damage that occurs during cold storage. Aliquots of 2 mL of diluted semen were stored either at 4°C or 10°C under anaerobic conditions, in the absence (control group) or presence of RSV at different concentrations (10, 20, 40, and 80 μM). Sperm quality parameters were assessed at 0 hours and after 24 hours of storage. Resveratrol treatment did not affect sperm quality parameters at 0 hours. At 24-hour storage, a significant (P < .01) decrease of sperm quality was observed independently from RSV supplementation and storage temperature. A significant decrease of viable spermatozoa with high mitochondrial membrane potential (SYBR+/PIâ/JC-1+) was evident at 24-hour storage in 40- and 80-μM RSV groups compared with control group. Moreover, a decline of total motility in 80-μM RSV group compared with the control group and a decrease of progressive motility and average path velocity in 80-μM RSV group compared with control and 20-μM RSV groups were observed. In conclusion, our findings demonstrate that RSV supplementation does not enhance sperm quality of stallion semen after 24 hours of storage. Moreover, 40- and 80-μM RSV concentrations could damage sperm functional status, probably acting as pro-oxidant. Finally, although 24-hour storage significantly affected most of the sperm quality parameters, no significant differences were found in groups maintained at 4°C or 10°C, suggesting that stallion semen could be equally preserved at these different temperatures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Equine Veterinary Science - Volume 34, Issues 11â12, NovemberâDecember 2014, Pages 1307-1312
Journal: Journal of Equine Veterinary Science - Volume 34, Issues 11â12, NovemberâDecember 2014, Pages 1307-1312
نویسندگان
Elisa DVM, Diego DVM, Gaetano DVM, Giovanna BS, Charles C. DVM, ACT, Carlo DVM, Marcella DVM,