کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8504649 | 1554982 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Benzo(a)pyrene suppresses tracheal antimicrobial peptide gene expression in bovine tracheal epithelial cells
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کلمات کلیدی
AHRLPSBeta-defensinsPAHGAPDHCYP1A1RT-qPCRBAPDMSO - DMSOInnate immunity - ایمنی ذاتیBenzo(a)pyrene - بنزو (a) پیرنهDimethyl sulfoxide - دیمتیل سولفواکسیدreverse transcription quantitative polymerase chain reaction - رونویسی معکوس واکنش زنجیره ای پلیمراز کمیEnvironmental toxicology - سم شناسی محیط زیستcytochrome P450 1A1 - سیتوکروم P450 1A1TAP - ضربه زدنCetaceans - فلفل دلمه ایlipopolysaccharide - لیپوپلی ساکاریدPolycyclic aromatic hydrocarbon - هیدروکربن آروماتیک چند حلقه ایPolycyclic aromatic hydrocarbons - هیدروکربن آروماتیک چندحلقهایCattle - گاوglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Respiratory disease is an important cause of morbidity and mortality in cetaceans, which are also threatened by environmental degradation caused by crude oil spills. Following oil spills, cetaceans at the water surface may inhale droplets of oil containing toxic polycyclic aromatic hydrocarbons (PAHs), which could potentially alter respiratory immunity via activation of the aryl hydrocarbon receptor (AHR) and its subsequent interaction with nuclear factor kappa B (NF-κB). β-defensins are antimicrobial peptides secreted by airway epithelial cells and their expression is known to be dependent on NF-κB. We hypothesized that PAHs may suppress the expression of β-defensins, and thereby contribute to the pathogenesis of pneumonia. This hypothesis was modeled by measuring the in vitro effects of benzo(a)pyrene (BAP), phenanthrene, and naphthalene on tracheal antimicrobial peptide (TAP) gene expression in bovine tracheal epithelial cells. Stimulation with lipopolysaccharide (LPS) induced 20â±â17-fold (meanâ±âSD) increased TAP gene expression. Exposure of tracheal epithelial cells to 5âμM BAP for 4 or 8âh, followed by incubation with a combination of LPS and 5âμM BAP for another 16âh, significantly (Pâ=â0.002) suppressed LPS-induced TAP gene expression by 40.6â±â21.8% (meanâ±âSD) in tracheal epithelial cells from 9 calves tested. BAP-induced suppression of TAP gene expression coincided with induction of cytochrome P450 1A1 gene expression. In contrast, phenanthrene and naphthalene had no consistent effect, and exposure to PAHs did not significantly affect constitutive TAP gene expression (i.e. without LPS). These findings characterize the suppressive effects of BAP-a toxic pollutant found in crude oil-on this respiratory innate immune response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 203, September 2018, Pages 40-46
Journal: Veterinary Immunology and Immunopathology - Volume 203, September 2018, Pages 40-46
نویسندگان
Laura A. Bourque, Stephen Raverty, Carmon Co, Brandon N. Lillie, Pierre-Yves Daoust, Mary Ellen Clark, Jeff L. Caswell,