کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8504691 1554984 2018 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment and characterization of a cell line from a feline histiocytic sarcoma
ترجمه فارسی عنوان
ایجاد و مشخص کردن یک سلول از یک سارکوم هیستوسیتیک کولی
کلمات کلیدی
سارکوم ویلیوتسیتیک جنین خط تلفن، تعیین مشخصات،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
Feline histiocytic sarcoma (HS) is an aggressive and uncommon tumor originating from dendritic cells/macrophages. Here, a feline HS cell line, FHS-1, was established from a case of feline HS and characterized. Immunohistochemically, FHS-1 cells were positive for vimentin and Iba-1, and negative for MHC class II and CD163. FHS-1 cells were positive for α-naphthyl butyrate esterase staining, which was clearly inhibited by sodium fluoride. FHS-1 cells had phagocytic and antigen uptake/processing activities. Moreover, FHS-1 cells were tested for susceptibility to feline infectious peritonitis virus (FIPV) strain 79-1146; however, this cell line was not susceptible to this viral strain. Although FHS-1 cells lost the expression of MHC class II and CD163, our findings indicate that FHS-1 is a feline HS cell line that retains functional properties of dendritic cells/macrophages in terms of phagocytic and antigen uptake/processing activities. While FHS-1 cells are not suitable for in vitro study of FIP using strain 79-1146, they may be applicable for studies aimed at developing new diagnostic and therapeutic strategies for feline HS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 201, July 2018, Pages 72-76
نویسندگان
, , , , , , , , , ,