کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644536 | 1569762 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Developmental characterization and environmental stress responses of Y-box binding protein 1 gene (AccYB-1) from Apis cerana cerana
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کلمات کلیدی
A30Apis cerana ceranaGRxdsRNATrxhsfCSDHspBR-CA15GSTCdxAGFPNCBIdouble-stranded RNA - RNA دو رشته ایHydrogen peroxide - آب اکسیژنهthioredoxin - تیرودوکسینcold shock domain - دامنه سرماخوردگیheat shock factor - عامل شوک گرماBroad-Complex - مجتمع مسکونیNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیH2O2 - هیدروژن پراکسیدHeat shock protein - پروتئین شوک حرارتgreen fluorescent protein - پروتئین فلورسنت سبزglutathione S-transferase - گلوتاتیون S-ترانسفرازGlutaredoxin - گلوتاراکسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Developmental characterization and environmental stress responses of Y-box binding protein 1 gene (AccYB-1) from Apis cerana cerana Developmental characterization and environmental stress responses of Y-box binding protein 1 gene (AccYB-1) from Apis cerana cerana](/preview/png/8644536.png)
چکیده انگلیسی
Y-box binding protein 1 (YB-1) is a member of the cold shock domain protein superfamily and is involved in development, environmental stresses and DNA oxidative damage in many organisms. However, the precise functions of YB-1 are still not well understood in various insects, including bees. In the current study, we identified a YB-1 gene in Apis cerana cerana (AccYB-1). The predicted cis-acting elements in the promoter sequence of AccYB-1 indicated its possible roles in development and stress responses. AccYB-1 expression was higher in one-day-old larvae and dark-eyed pupae than in other development stages. Tissue-specific expression analysis showed that the mRNA level of AccYB-1 was higher in the thorax and midgut than in other tissues. The results from real-time PCR showed that AccYB-1 was induced by many environmental stresses. Silencing AccYB-1 downregulated the transcriptional level of some growth- and development-related genes and antioxidant genes and decreased the enzyme activities of several antioxidant-related enzymes, further indicating a possible function of AccYB-1 in growth, development and stress responses. Taken together, our findings suggest that AccYB-1 may play an indispensable role in growth and development and environmental stress responses in Apis cerana cerana. To our knowledge, this is the first paper to explore the role of YB-1 in bees.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 674, 20 October 2018, Pages 37-48
Journal: Gene - Volume 674, 20 October 2018, Pages 37-48
نویسندگان
Guilin Li, Lijun Wang, Ying Wang, Han Li, Zhenguo Liu, Hongfang Wang, Baohua Xu, Xingqi Guo,