کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644665 | 1569765 | 2018 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx
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کلمات کلیدی
IEGsN-methyl-d-asparateCynopterus sphinx2-DGEAS-ODNhypoxanthine phosphoribosyltransferase-1NMDAMCLLC-MS/MS - LC-MS / MS2-Dimensional gel electrophoresis - الکتروفورز ژل دو بعدیIHC - ایمونوهیستوشیمیmiR-132 - به miR-132immunohistochemical analysis - تجزیه و تحلیل ایمونوهیستوشیمیSERT - سختserotonin transporter - سروتونین حمل کنندهLiquid chromatography-mass spectrometry - طیف سنجی جرم کروماتوگرافی مایعmitral cell layer - لایه سلولی میترالolfactory bulb - پیاز بویاییImmediate early genes - ژنهای سریع و سریعNMDA receptors - گیرنده NMDAOlfactory learning - یادگیری لذت بخش
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx](/preview/png/8644665.png)
چکیده انگلیسی
Earlier, we showed that micro RNA-132 (miR-132) regulate the immediate early genes (IEGs) in the olfactory bulb (OB) of fruit bat Cynopterus sphinx during olfactory learning. This study was designed to examine whether the miR-132 regulate other proteins in OB during olfactory learning. To test this, miR-132 anti-sense oligodeoxynucleotide (AS-ODN) was delivered to the OB and then trained to novel odor. The 2-dimensional gel electrophoresis analysis showed that inhibition of miR-132 altered olfactory training induced expression of 321 proteins. Further, liquid chromatography-mass spectrometry (LC-MS/MS) analysis reveals the identity of differently expressed proteins such as phosphoribosyl transferase domain containing protein (PRTFDC 1), Sorting nexin-8 (SNX8), Creatine kinase B-type (CKB) and Annexin A11 (ANX A11). Among them PRTFDC 1 showing 189 matching peptides with highest sequence coverage (67.0%) and protein-protein interaction analysis showed that PRTFDC 1 is a homolog of hypoxanthine phosphoribosyltransferase-1 (HPRT-1). Subsequent immunohistochemical analysis (IHC) showed that inhibition of miR-132 down-regulated HPRT expression in OB of C. sphinx. In addition, western blot analysis depicts that HPRT, serotonin transporter (SERT), N-methyl-d-asparate (NMDA) receptors (2A,B) were down-regulated, but not altered in OB of non-sense oligodeoxynucleotide (NS-ODN) infused groups. These analyses suggest that miR-132 regulates the process of olfactory learning and memory formation through SERT and NMDA receptors signalling, which is possibly associated with the PRTFDC1-HPRT interaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 671, 10 September 2018, Pages 10-20
Journal: Gene - Volume 671, 10 September 2018, Pages 10-20
نویسندگان
Murugan Mukilan, David Mary Rajathei, Edwin Jeyaraj, Nagarajan Kayalvizhi, Koilmani Emmanuvel Rajan,