کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644706 | 1569766 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adipose-specific expression of mouse Rbp7 gene and its developmental and metabolic changes
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کلمات کلیدی
RT-PCRIWATPPARγDlk1BATFABP4qPCRCYCUcp1 - UCP1inguinal white adipose tissue - بافت چربی سفید گوشتیbrown adipose tissue - بافت چربی قهوه ایTics - تیکسAlternative splicing - جابجایی جایگزینRetinoic acid - رتینوئیک اسیدRetinol - رتینولROL - رولFat cell - سلول چربیcyclophilin - سیکلوفیلینCold exposure - قرار گرفتن در معرض سردreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیfatty acid binding protein 4 - پروتئین اتصال دهنده اسید چرب 4uncoupling protein 1 - پروتئین جدا سازی 1Peroxisome proliferator-activated receptor-γ - گیرنده پروتئینی فعال پروکسیوم - γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Alternative splicing and alternative promoter usage have been shown to have an integral role in creating flexibility in the regulation of gene expression. Previous studies collectively showed that expression of the retinol binding protein 7 (Rbp7) gene was adipose tissue-specific across species. Nevertheless, alternative splicing and alternative promoter usage of the Rbp7 gene in adipose tissue and other tissues have not been investigated. The objectives of this study were to investigate protein isoforms of RBP7 produced from alternative splicing, alternative promoter usage and pre-mRNA trans-splicing, and to examine expression patterns of RBP7 isoforms during adipogenesis, cold exposure, and retinol or retinoic acid treatment. Our RT-PCR analysis revealed that mouse Rbp7 isoforms were present, but only one protein isoform was detected which was specific to adipose tissue. In addition, a fusion transcript of the Nmnat1 gene and the Rbp7 gene was produced by pre-mRNA trans-splicing in several tissues; however, its protein expression was not detectable. During adipogenesis, RBP7 expression was prominent in both neonatal and after-weaning stages and its expression was significantly higher in fat cells than in preadipocytes. Exposure to cold led to an increased expression of RBP7 in brown adipose tissue (BAT). Furthermore, Rbp7 mRNA expression in 3T3-L1 cells was significantly up- and down-regulated by retinol and retinoic acid, respectively. Our data showed that the mouse Rbp7 gene produces a predominant isoform in adipose tissue during adipocyte development, cold exposure, and nutritional treatments, which can be a potential target for future investigation on reduced adiposity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 670, 5 September 2018, Pages 38-45
Journal: Gene - Volume 670, 5 September 2018, Pages 38-45
نویسندگان
Jinsoo Ahn, Dong-Hwan Kim, Yeunsu Suh, Jeong-Woong Lee, Kichoon Lee,