کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645291 | 1569780 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polymorphism in promoter of SIX4 gene shows association with its transcription and body measurement traits in Qinchuan cattle
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IFCQinchuan cattleSNPsGLMULAPICQTLqPCRchest depthbody measurement traitsMAS - بیشترQuantitative trait loci - تعداد صفات صفاتback fat thickness - ضخامت چربی پشتbody length - طول بدنwithers height - قد بلندpolymorphism information content - محتوای اطلاعات پلی مورفیسمIntramuscular fat content - محتوای چربی داخل عضلانیChest circumference - محدوده قفسه سینهGeneral linear models - مدل خطی عمومیquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیSingle nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Polymorphism in promoter of SIX4 gene shows association with its transcription and body measurement traits in Qinchuan cattle Polymorphism in promoter of SIX4 gene shows association with its transcription and body measurement traits in Qinchuan cattle](/preview/png/8645291.png)
چکیده انگلیسی
The sine oculis homeobox homolog 4 (SIX4) gene belongs to the SIX gene family, which plays a critical role in muscle regeneration and early stages of ontogeny. This study aimed to detect promoter variations of bovine SIX4 genes in Qinchuan cattle, and to evaluate the effect of transcription regulations and body measurement traits. Quantitative real-time PCR (qPCR) results showed that the mRNA expression levels of SIX4 gene were found significantly highest in longissimus thoracis tissue and individual before attaining the stage of physiological maturity. Using sequencing technology on a total of 428 Qinchuan cattle, seven single nucleotide polymorphisms (SNPs) were identified in the promoter region of SIX4, and seven haplotypes representing 18 potential transcription factor compositions of polymorphic potential cis-acting elements. Association analysis indicated that the H3-H3 diplotype performed greater withers height, chest depth, chest circumference, back fat thickness and ultrasound loin muscle area (Pâ¯<â¯0.05) than H5-H6, which were consistent with the promoter activity of Hap3 haplotype was higher than the Hap5 and Hap6 haplotype in vitro. These potential transcription factor information and combined genotypes H3-H3 of the SIX4 gene can be used as a molecular marker for selection of economic traits in Qinchuan cattle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 656, 20 May 2018, Pages 9-16
Journal: Gene - Volume 656, 20 May 2018, Pages 9-16
نویسندگان
Dawei Wei, Sayed Haidar Abbas Raza, Jiupan Zhang, Linsheng Gui, Siddiq Ur Rahman, Rajwali Khan, Seyed Mahdi Hosseini, Hubdar Ali Kaleri, Linsen Zan,