کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645742 | 1569791 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GmPLP1, a PAS/LOV protein, functions as a possible new type of blue light photoreceptor in soybean
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کلمات کلیدی
qRT-PCRPhyShort daysMurashige and Skoog - Murashige و SkoogPer-Arnt-Sim - per-arnt-simArabidopsis thaliana - آرابیدوپسیس تالیاناlong days - روزهای طولانیreal-time quantitative PCR - زمان واقعی PCR کمیCircadian Clock - ساعت دیجیتالPhytochrome - فیتوکرومLOV - قانونPAS - نهBlue light - نور آبیCry - گریه کردن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Light is one of the most important environmental factors for the growth and development of plants. To adapt to changes in day length, the photoreception and transmission of the light signals in plants mainly depend on the various light receptor proteins. The PAS/LOV protein (PLP) has a PAS domain in the N-terminal and LOV domain in the C-terminal and has been confirmed as a new type of blue light receptor in Arabidopsis thaliana. However, the role of its counterpart in soybean remains largely unclear. In this study, the expression pattern of the GmPLP1 under different light qualities was determined by real-time RT-PCR analysis using the cultivar 'DongNong 42', a photosensitive soybean cultivar, suggesting that GmPLP1 was affected by the circadian clock and was a dark-induced gene. Moreover, the mRNA abundance increased significantly under blue light. Further analysis revealed that overexpression of GmPLP1 displayed the inhibition of hypocotyl elongation under blue light, and the expression of CRY1, CRY2, CKL3, CKL4, BIT1, and HY5 were simultaneously increased in GmPLP1-transgenic Arabidopsis, suggesting that the shortened hypocotyl was associated with the up-regulation of these genes. Taken together, our results suggest that GmPLP1, which is a new possible type of blue light photoreceptor in soybean, plays an important role in the blue light signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 645, 1 March 2018, Pages 170-178
Journal: Gene - Volume 645, 1 March 2018, Pages 170-178
نویسندگان
Yongguang Li, Yanzheng Zhang, Mengting Li, Qiulan Luo, Ali Inayat Mallano, Ya Jing, Yuhang Zhang, Lin Zhao, Wenbin Li,