کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646011 | 1569794 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ClC-3 chloride channel is involved in isoprenaline-induced cardiac hypertrophy
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کلمات کلیدی
Intraperitoneal injectionsDMEMFBSANFAAV9S.E.M.BSA - BSACVDs - CVD هاH&E - H & EDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccobovine serum albumin - آلبومین سرم گاوIsoprenaline - ایسوپرنالینleft ventricle - بطن چپCardiovascular diseases - بیماری قلبی-عروقی standard error of the mean - خطای استاندارد میانگینfetal bovine serum - سرم جنین گاوHematoxylin and Eosin - هماتوکسیلین و ائوزینCardiac hypertrophy - هیپرتروفی قلبیChloride channel - کانال کلرایدejection fraction - کسری خروجیfractional shortening - کوتاه کردن کسری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Isoprenaline, an activator of β-adrenergic receptor, has been found to induce cardiac hypertrophy in vivo and in vitro, but the exact mechanism is still unclear. ClC-3 is a member of the chloride channel family and is highly expressed in mammalian myocardium. In the present study, the role of ClC-3 in isopronaline-induced cardiac hypertrophy was investigated. We found that ClC-3 expression was reduced in isoprenaline-induced hypertrophic H9c2 cells, primary rat neonatal cardiomyocytes and myocardium of C57/BL/6 mice, and this reduction was prevented by the pretreatment of propranolol. Adeno-associated virus 9 (AAV9)-mediated ClC-3 expression in myocardium decreased heart mass index, thinned interventricular septum and left ventricular wall and lowered the mRNA expression of natriuretic peptide type A (ANF) and β-myosin heavy chain (β-MHC). Our results showed that ClC-3 played an important role in β-adrenergic cardiac hypertrophy which could be associated with ANF and β-MHC, and all these findings suggested that ClC-3 may be a novel therapeutic target for the prevention or treatment of myocardiac hypertrophy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 642, 5 February 2018, Pages 335-342
Journal: Gene - Volume 642, 5 February 2018, Pages 335-342
نویسندگان
Chunmei Li, Dan Huang, Jing Tang, Mengqing Chen, Qun Lu, He Li, Mengzhen Zhang, Bin Xu, Jianwen Mao,