کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8846701 | 1617758 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Long-term fertilization changes bacterial diversity and bacterial communities in the maize rhizosphere of Chinese Mollisols
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The rhizosphere is a dynamic interface in which interactions among a myriad of microorganisms affect plants growth and tolerance to biotic and abiotic stress. Although rhizosphere effects on soil microbial communities have been widely investigated, few studies have evaluated such impacts of long-term fertilization on rhizosphere microbial communities in black soils common to northeast China. Here, we applied quantitative real-time polymerase chain reaction and high-throughput pyrosequencing to characterize rhizosphere and bulk soil bacterial communities in a long-term (36-year) fertilizer experiment. Soils were subjected to six treatments: CK (no fertilizer), N1 (150â¯kg ureaâ¯haâ1â¯yâ1), N2 (300â¯kg ureaâ¯haâ1â¯yâ1), M (18,600â¯kg horse manureâ¯haâ1â¯yâ1), NPK (150â¯kg urea plus 33â¯kg P plus 62â¯kgâ¯Kâ¯haâ1â¯yâ1), and MNPK (M plus NPK). Inorganic fertilizer, especially N, decreased the 16S rRNA gene copy numbers and bacterial diversity in the rhizosphere and bulk soil, while manure fertilizer increased these values. Moreover, 16S rRNA gene copy numbers were higher and bacterial diversity was lower in the rhizosphere than the bulk soil, indicating that the maize rhizosphere had significant effects on bacterial diversity. The bacterial communities were predominantly composed of Proteobacteria and Acidobacteria in both the rhizosphere and bulk soil, but the rhizosphere and bulk soil communities were distinguished by principal coordinates analysis. Soil pH correlated with bacterial community composition and diversity in both rhizosphere and bulk soil. However, bacterial community composition in rhizosphere was more correlated with soil nutrient concentrations than in bulk soil under long-term fertilization. A redundancy analysis also indicated that soil pH, organic matter and available phosphorus concentrations were the most important factors in shaping bacterial communities in the maize rhizosphere. Our results revealed that long-term fertilization with increasing nutrients availability increased bacterial abundance, decreased biodiversity and changed bacterial composition in the rhizosphere.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Soil Ecology - Volume 125, April 2018, Pages 88-96
Journal: Applied Soil Ecology - Volume 125, April 2018, Pages 88-96
نویسندگان
Qingfeng Wang, Xin Jiang, Dawei Guan, Dan Wei, Baisuo Zhao, Mingchao Ma, Sanfeng Chen, Li Li, Fengming Cao, Jun Li,