کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8877692 1624213 2018 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in mycoparasite-Fusarium hosts interfaces in response to hostile environment as revealed by water contact angle and atomic force microscopy
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Changes in mycoparasite-Fusarium hosts interfaces in response to hostile environment as revealed by water contact angle and atomic force microscopy
چکیده انگلیسی
The aim of this study is to characterize the mycoparasitic attributes of fungal surface hydrophobicity and topography influenced by nutrient conditions and dryness in Sphaerodes-Fusarium dual culture assays. This study presents detailed methods to study fungal cell surface texture or roughness under atomic force microscopy (AFM) and hydrophobicity measured by water contact angle (WCA). The differential expression of the WCA depicted a hydrophilic nature of S. mycoparasitica mycoparasite compared to highly hydrophobic F. proliferatum and low hydrophobic F. redolens plant pathogens showing antagonistic and biotrophic interactions, respectively. A shift in WCA induced by potato dextrose agar (PDA) versus nitrogen rich synthetic-ICI medium greatly affected a level of mycoparasitism as measured by the changes in fungal radial growth. The AFM portrayed differential changes in cell wall topography with possible cell-cell adhesion when exposed to desiccation. These findings propose that changes in fungal cell wall topography and hydrophobicity may foster more/less conductive microenvironment for mycoparasitism as related to physicochemical properties of the fungus-fungus interfaces. Furthermore, hostile environment exposed to dryness and/or limited nutrient supply may dictate the effectiveness of the mycoparasite in controlling plant pathogenic Fusarium hosts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biological Control - Volume 121, June 2018, Pages 247-255
نویسندگان
, ,