کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8961140 | 1646468 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An efficient colorimetric high-throughput screening method for synthetic activity of tyrosine phenol-lyase
ترجمه فارسی عنوان
یک روش غربالگری کارآمد برای اندازه گیری اثر سنتتیک تریروزین فنول لیاز
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کلمات کلیدی
غربالگری بالا، تریروزین فنل لیاز، فعالیت مصنوعی، واکنش کلریمتریک،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Tyrosine phenol-lyase (TPL) naturally catalyzes the reversible β-elimination of l-tyrosine to phenol, pyruvate and ammonium. With its reverse reaction (synthetic activity), l-tyrosine and its derivatives could be synthesized with high atom economy, which are widely used in pharmaceutical industries. In this study, a high-throughput screening method for synthetic activity of TPL was developed. One of the substrate, sodium pyruvate was found to react with salicylaldehyde under alkali condition, forming a yellow color compound. The concentration of sodium pyruvate can be quantified according to the absorbance of the colorimetric compound at wavelength of 465â¯nm and the activity of TPL could be screened according to the decrease of the absorbance. After optimization of the colorimetric reaction conditions, the established high-throughput screening method was successfully used for screening of TPL with enhanced activity for l-DOPA synthesis. The confirmed sensitivity and accuracy demonstrated the feasibility and application potential of this screening method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 560, 1 November 2018, Pages 7-11
Journal: Analytical Biochemistry - Volume 560, 1 November 2018, Pages 7-11
نویسندگان
Xiao-Ling Tang, Hui Suo, Ren-Chao Zheng, Yu-Guo Zheng,