کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8961141 | 1646468 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acetylcholinesterase biosensor based on functionalized surface of carbon nanotubes for monocrotophos detection
ترجمه فارسی عنوان
بیوسنسور استیل کولین استراز بر اساس سطح عملکردی نانولوله های کربنی برای تشخیص تک کروتوفوس
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کلمات کلیدی
مونوکروتوفوس، استیل کولین استراز، الکترود آنزیم، نانولوله های کربنی، اصلاح سطح،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Monocrotophos (Ops) has been widely used as pesticide in crop production but simultaneously could accumulate in the nature and seriously impact food safety and human health. It is necessary to develop a high sensitivity biosensor for accurate and fast detection of OPs. In this study, multi-walled carbon nanotubes (MWCNTs) were selected as acetylcholinesterase (AChE) carrier and their surface was modified by introducing different functional groups (-SH, -NH2, -Cl, -OH), hydrophobic alkyl groups (-CH3, -(CH2)2CH3, -(CH2)7CH3, -(CH2)15CH3) and ionic liquids (-IL1, -IL2). The interaction mechanism of MWCNTs functionalized surface and AChE has been revealed by studying characteristics of AChE immobilized on different carrier surface. Finally, compared with reported references and above other modifiers, we found that MWCNTs surface modified by -IL1 was the best carrier for AChE and the detection limit of IL1-MWCNTs/AChE/GCE was 3.3â¯Ãâ¯10â11â¯M. At optimum reaction condition (pH 7.0, AChE loading 0.25 U, Inhibition time 14â¯min), storability test indicated reactivity of IL1-MWCNTs/AChE/GCE remained above 98.5% within two weeks. For real vegetable sample detection, the recoveries of IL1-MWCNTs/AChE/GCE were found to be between 90.0% and 104%. These results demonstrated novel biosensors could act as device of high sensitivity for accurate and fast detection of OPs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 560, 1 November 2018, Pages 12-18
Journal: Analytical Biochemistry - Volume 560, 1 November 2018, Pages 12-18
نویسندگان
Zou Bin, Chu Yanhong, Xia Jiaojiao, Yao Jing,