کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8967118 | 1101381 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ontogenetic development of mRNA levels and binding sites of hepatic β-adrenergic receptors in cattle
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Catecholamines regulate glucose metabolism and affect hepatic glucose production mainly through β2-adrenergic receptors. The hypothesis was tested that gene expression and numbers of hepatic β-adrenergic receptors in calves are influenced by age. Examined developmental stages included pre-term (P0) and full-term (F0) calves immediately after birth, full-term calves on day 5 of life (F5), and veal calves (VC) at the age of 159 days. Expression of β1-, β2-, and β3-adrenergic receptor mRNA was measured by real-time PCR. Receptor binding was quantified by saturation binding assays using (3H)-CGP-12177 as a ligand. Abundance of mRNA differed among β-adrenergic subtypes (β2 > β1 > β3; P < 0.01). β3-Adrenergic receptor mRNA was undetectable in VC. mRNA abundance for β2-adrenergic receptors was higher (P < 0.05) in VC than P0 and for β3-adrenergic receptors was higher (P < 0.001) in F5 than P0. Binding studies revealed most binding of (3H)-CGP-12177 to β2-adrenergic receptors, which were highest in VC (P < 0.001) and higher (P < 0.05) in F5 than P0. Binding sites correlated positively with mRNA levels of β2-adrenergic receptors (r = 0.67; P < 0.001), with hepatic activities of phosphoenolpyruvate kinase (r = 0.73; P < 0.001) and with pyruvate kinase (r = 0.4; P < 0.05), and with plasma glucose concentrations (r = 0.5; P < 0.01). In conclusion, mRNA of all three β-adrenergic receptor subtypes were found in liver, with β2-adrenergic receptors being the dominant subtype. Numbers of β2-adrenergic receptors increased with age and were mainly regulated at the transcriptional level. Numbers of β-adrenergic receptors were positively associated with hepatic activities of gluconeogenetic enzymes and with plasma glucose levels, suggesting functional importance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Domestic Animal Endocrinology - Volume 28, Issue 3, April 2005, Pages 320-330
Journal: Domestic Animal Endocrinology - Volume 28, Issue 3, April 2005, Pages 320-330
نویسندگان
J. Carron, C. Morel, H.M. Hammon, J.W. Blum,