کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8967236 | 1101402 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hormonal regulation of leptin receptor expression in primary cultures of porcine hepatocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
A study was conducted to elucidate hormonal control of leptin receptor gene expression in primary cultures of porcine hepatocytes. Hepatocytes were isolated from pigs (52Â kg) and seeded into collagen-coated T-25 flasks. Monolayer cultures were established in medium containing fetal bovine serum for 1 day and switched to a serum-free medium for the remainder of the 3-day culture period. To establish basal conditions hepatocytes were maintained in serum-free William's E medium containing 10Â nM dexamethasone and 1Â ng/ml insulin. For the final 24Â h, insulin (1 or 100Â ng/ml) or glucagon (100Â ng/ml), were added in the presence or absence of 100Â nM triiodothyronine (T3). RNA was extracted and quantitative RT-PCR was performed with primers specific for the long form and total porcine leptin receptors. Leptin receptor expression was calculated relative to co-amplified 18S rRNA. Expression of the long form of the leptin receptor was confirmed under basal conditions. Insulin, glucagon and synthetic human proteins (ghrelin and GLP-1) at 100Â ng/ml had no influence on leptin receptor expression; the addition of T3 was associated with a marked increase (PÂ <Â 0.001) in expression of total and long forms of the leptin receptor by 1.6 and 2.4-fold, respectively. Addition of leptin to cells which were pre-treated with T3 for 24Â h (to up-regulate leptin receptor expression), confirmed the lack of a direct effect of leptin on glucagon-induced glycogen turnover and cAMP production. These data suggest that porcine hepatocytes may be insensitive to leptin stimulation even when leptin receptor expression is enhanced by T3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Domestic Animal Endocrinology - Volume 29, Issue 4, November 2005, Pages 582-592
Journal: Domestic Animal Endocrinology - Volume 29, Issue 4, November 2005, Pages 582-592
نویسندگان
T.J. Caperna, A.E. Shannon, S.M. Poch, W.M. Garrett, M.P. Richards,