کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987742 | 1555132 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of three assays for the evaluation of specific cellular immunity to Leishmania infantum in dogs
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Cellular immune response to Leishmania plays a key role in canine leishmaniosis. However, there are few assays to evaluate this response in the dog. Here, we evaluated and compared three assays of specific cellular immune response to Leishmania infantum in dogs: the leishmanin skin test (LST), lymphocyte proliferation assay (LPA) and IFN-γ cytopathic effect inhibition bioassay (IFNB). Fifty-six healthy dogs from an endemic area for leishmaniosis on the island of Mallorca were studied. In all, 37 dogs showed a positive LST and 32 a positive IFNB, 24 were positive for both assays. The 17 dogs positive for LPA also gave positive results for either LST or IFNB, or both. These findings indicate that although LST is the method of choice, IFNB is a complementary test. Therefore, both assays should be performed and analyzed jointly. In comparison with LST and IFNB, LPA is much less sensitive, and yields many false negative results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 107, Issues 1â2, 15 August 2005, Pages 163-169
Journal: Veterinary Immunology and Immunopathology - Volume 107, Issues 1â2, 15 August 2005, Pages 163-169
نویسندگان
Hugo Fernández-Bellon, Laia Solano-Gallego, Alhelà RodrÃguez, Victor PMG Rutten, Aad Hoek, Antonio Ramis, Jordi Alberola, LluÃs Ferrer,