کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987795 | 1555137 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR](/preview/png/8987795.png)
چکیده انگلیسی
A monoclonal antibody blocking enzyme-linked immunosorbent assay (B-ELISA) was developed to detect antibodies to Mycoplasma bovis in cattle sera. The assay was highly specific and sensitive and there was no cross-reaction detected. This method revealed a high prevalence of antibodies (60%) to M. bovis in dairy cattle in North Queensland. The diagnostic potential of this B-ELISA for the detection of antibody to M. bovis was compared with its detection by PCR. There was a strong positive correlation between PCR and B-ELISA titers. Thus, the B-ELISA appears to be a valuable and reproducible tool in the serodiagnosis of M. bovis infection in cattle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 104, Issues 3â4, 8 April 2005, Pages 183-193
Journal: Veterinary Immunology and Immunopathology - Volume 104, Issues 3â4, 8 April 2005, Pages 183-193
نویسندگان
Ali Ghadersohi, Zahra Fayazi, Robert G. Hirst,