کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987863 | 1555140 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and characterization of a homologue of the alpha inhibitor of NF-κB in Rainbow trout (Oncorhynchus mykiss)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
A homologue of IκBα, the alpha member of the IκB family of NF-κB inhibitors, was identified in a Rainbow trout suppression subtractive hybridization library enriched in sequences up-regulated in cultured leukocytes after lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNFα) stimulation. The full-length cDNA was isolated and sequenced. The predicted amino acid sequence is 61.5% similar and 54% identical to human IκBα, while only 42% similar and 35% identical to IκBβ, and 38% similar and 32% identical to IκBÉ. Rainbow trout IκBα contains a central ankyrin repeat domain required for its interaction with NF-κB and a putative PEST-like sequence in the C-terminus. Expression of IκBα is up-regulated by LPS and TNFα treatment, two known activators of NF-κB, suggesting the existence of an autoregulatory loop in fish, as is the case for mammals. These results confirm the existence of the NF-κB signalling pathway in fish and suggest a similar functional interaction between IκBα and NF-κB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 103, Issues 1â2, 10 January 2005, Pages 1-7
Journal: Veterinary Immunology and Immunopathology - Volume 103, Issues 1â2, 10 January 2005, Pages 1-7
نویسندگان
A. Sangrador-Vegas, T.J. Smith, M.T. Cairns,