کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8987873 1555140 2005 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of new monoclonal antibodies against porcine lymphocytes: molecular characterization of clone 7G3, an antibody reactive with the constant region of the T-cell receptor δ-chains
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Characterization of new monoclonal antibodies against porcine lymphocytes: molecular characterization of clone 7G3, an antibody reactive with the constant region of the T-cell receptor δ-chains
چکیده انگلیسی
A battery of mouse monoclonal antibodies (mAbs) reactive with porcine peripheral blood (PB) leukocytes was generated. Among the mAbs, 6F10 was found to react probably with cluster of differentiation (CD)8 α-chain, while 7G3 and 3E12 were found to recognize γδ T-cells, as revealed by two-color flow cytometric and immunoprecipitation studies. 7G3 was shown to react with the constant (C) region of the T-cell receptor (TCR) δ-chain by the following facts: (1) 7G3 immunoprecipitated full-length TCR δ-chain protein fused with glutathione S-transferase (GST) produced by Esherichia coli and (2) 7G3 reacted with TCR δ-chain expressing Cos-7 cells transfected with either full-length or N-terminal deleted mutant cDNA, but did not react with Cos-7 cells transfected with C-terminal deleted mutant TCR δ-chain cDNA. All three mAbs produced high-quality immunostaining results on frozen sections, revealing a distinct distribution of γδ T-cells and CD8+ cells. This report precisely characterizes mAbs against porcine TCR for the first time, facilitating molecular biological investigations of the porcine immune system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 103, Issues 1–2, 10 January 2005, Pages 113-127
نویسندگان
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