کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9103465 1152802 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of aging on the relation of adenyl purine release with plasma membrane fluidity of arterial endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Effects of aging on the relation of adenyl purine release with plasma membrane fluidity of arterial endothelial cells
چکیده انگلیسی
Age-related changes in adenyl purine release from rat arteries and endothelial cell (EC) plasma membrane (PM) fluidity were studied. High performance liquid chromatography-fluorescence revealed that aging significantly decreased the release of adenyl purines. Pyrene-excimer spectroscopy disclosed that EC PM fluidity of aged rats decreased more significantly than that of young rats. An increase in cholesterol content and a decrease in the unsaturation index (USI) of fatty acids in cholesterol-enriched ECs reduced PM fluidity and 5′-nucleotidase (5′-ND) activity (measured by coupled assay of adenosine deaminase and glutamate dehydrogenase). Moreover, a decrease in cholesterol content and an increase in the USI of fatty acyl chains of the PM in docosahexaenoic acid-enriched ECs concurrently increased enzyme activity and extracellular adenosine. Therefore, decreases in PM fluidity, observed with age-dependent increased cholesterol and decreased USI, induce a decrease in 5′-ND activity, decrease extracellular adenosine levels, and might relate to hypertension in aged rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids - Volume 73, Issue 6, December 2005, Pages 475-483
نویسندگان
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