Role of peroxynitrite in the process of vascular tone regulation by nitric oxide and prostanoids-a nanotechnological approach

ONOO− decreased PGI2 activity with IC50≈150 nmol/L for 8 min reaction time, but has no effect of short reaction time. Prostaglandin E1 decreased NO, O2−, and ONOO− by limiting Ca2+ flux into endothelium, decreased edema and vasoconstriction during ischemia/reperfusion. In endothelium (HUVEC's) of Black's the ONOO− concentrations were high 750±50 nmol/L while the lowest concentrations of vasorelaxants were 275±25 nmol/L of NO, 150±15 pb/100 μg protein of 6-keto-PGF1α as compared to White's (420±30 and 470± nmol/L for ONOO− and NO respectively and 280±20 pg/100 mg protein for 6-keto-PGF1α).