کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9126852 | 1569963 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth
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کلمات کلیدی
ADCdcSAMdecarboxylated S-adenosylmethionineACL5SPDSSAMDCODCOrnithine decarboxylaseSPMSCaMVSPDDAFSPMPCA - PCAS-adenosylmethionine decarboxylase - S-adenosylmethionine dearboxylasearginine decarboxylase - آرژینین دکربوکسیلازSpermine - اسپرمSpermidine - اسپرمیدینSpermidine synthase - اسپنتیدین سنتازPerchloric acid - اسید پرکلریکrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدdays after full bloom - روز بعد از شکوفه کاملSpermine synthase - سنتاز اسپرمDIG - شماputrescine - قرار دادنPUT - قرار دادنRace - مسابقهcauliflower mosaic virus - ویروس موزائیک گل کلمPolyamine - پلی آمین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth](/preview/png/9126852.png)
چکیده انگلیسی
Three cDNAs with high homology to spermine (Spm) synthases in Arabidopsis were isolated from apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.]. MdACL5-1 and MdACL5-2 have high homology with ACL5 and MdSPMS has high homology with AtSPMS. The similarity of MdSPMS to spermidine synthases (SPDSs) was higher than that of MdACL5s, despite the fact that both are putative Spm synthases. However, MdSPMS could be discriminated from SPDSs by the presence of several characteristic amino acids, i.e., Val-149, Ser-161, Ala-205, and Val-235, in the decarboxylated S-adenosylmethionine (dcSAM)-binding motif of MdSPMS. Both MdACL5-1 and MdSPMS complemented Spm biosynthesis in a yeast mutant deficient in Spm synthase, and ectopic expression of MdACL5-1 in the Arabidopsis dwarf mutant acl5 allowed recovery of the normal phenotype. RNA gel blot analysis showed that MdACL5 and MdSPMS are differentially expressed in tissues and suspension cells. These results suggest that functional MdACL5 and MdSPMS are independently involved in apple fruit development and cell growth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 361, 21 November 2005, Pages 101-111
Journal: Gene - Volume 361, 21 November 2005, Pages 101-111
نویسندگان
Hiroyasu Kitashiba, Yu-Jin Hao, Chikako Honda, Takaya Moriguchi,