کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9126874 | 1569961 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The mouse sphingomyelin synthase 1 (SMS1) gene is alternatively spliced to yield multiple transcripts and proteins
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کلمات کلیدی
PBSDMEMNaOHoligodeoxyribonucleotidePARPGFPkiloDaltonSAMHRPkDacDNA - cDNART–PCR - RT-PCRSDS–PAGE - SDS-PAGEoligo - الیگوimmunoglobulin - ایمونوگلوبولینBase pair(s) - جفت پایه (ها)diacylglycerol - دیسیل گلیسیرینDulbecco's modified Eagle's media - رسانه های عقاب اصلاح شده DulbeccoDAG - روزPhosphate buffered saline - فسفات بافر شورsterile alpha motif - مورچه آلفا استریلsodium hydroxide - هیدروکسید سدیمreverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسhorse radish peroxidase - پراکسیداز تربچه اسبgreen fluorescent protein - پروتئین فلورسنت سبزPlasmid - پلاسمیدPoly(ADP-ribose) polymerase - پلیمر (ADP-ribose) پلیمرازGal - گالGalactose - گالاکتوزمی Glu - گلوGlucose - گلوکز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sphingomyelin synthase 1 (SMS1) is a recently identified 413-residue protein that plays a critical role in sphingolipid metabolism by catalyzing the conversion of ceramide and phosphatidylcholine to sphingomyelin and diacylglycerol (DAG). We have previously reported the isolation of a mouse SMS1 encoding cDNA that contains a unique 5ⲠUTR sequence. Three other mouse SMS1 cDNAs that differed in their 5Ⲡand 3Ⲡnon-coding sequences were present in GenBank. In order to ascertain the origin of the unique 5Ⲡand 3ⲠUTR sequences, we analyzed the structure of the mouse SMS1 gene. Analysis of the four different SMS1 cDNA sequences and of the corresponding mouse genomic fragment revealed that the SMS1 gene consists of 16 exons that are alternatively spliced to produce 4 different mRNAs (SMS1α1, SMS1α2, SMS1β and SMS1γ) and 3 different proteins (SMS1α, SMS1β and SMS1γ). RT-PCR was used to demonstrate that all four SMS1 cDNAs represent expressed transcripts that show distinctly different tissue distributions. Transcripts for SMS1α1, SMS1α2 and SMS1β were found to increase in response to the pro-apoptotic effects of TNF-α. Finally, using a yeast-based assay, we confirmed that SMS1α prevents the growth inhibitory effects of Bax but SMS1β neither prevents nor enhances the effects of Bax or of SMS1α. Taken together these results demonstrate the complexity of SMS1 gene structure, expression and function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 363, 19 December 2005, Pages 123-132
Journal: Gene - Volume 363, 19 December 2005, Pages 123-132
نویسندگان
Zhao Yang, Gaël Jean-Baptiste, Chamel Khoury, Michael T. Greenwood,