کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9144628 | 1164526 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polygalacturonase-inhibiting protein 2 of Phaseolus vulgaris inhibits BcPG1, a polygalacturonase of Botrytis cinerea important for pathogenicity, and protects transgenic plants from infection
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Polygalacturonase-inhibiting protein 2 of Phaseolus vulgaris inhibits BcPG1, a polygalacturonase of Botrytis cinerea important for pathogenicity, and protects transgenic plants from infection Polygalacturonase-inhibiting protein 2 of Phaseolus vulgaris inhibits BcPG1, a polygalacturonase of Botrytis cinerea important for pathogenicity, and protects transgenic plants from infection](/preview/png/9144628.png)
چکیده انگلیسی
The genome of the necrotrophic fungus Botrytis cinerea encodes at least six endopolygalacturonases; among them BcPG1 is constitutively expressed and is required for full virulence. To counteract fungal polygalacturonase, plants have evolved families of polygalacturonases-inhibiting proteins (PGIPs) with specific recognition capabilities against PGs from different fungal species. However, it is not clear whether PGIPs can specifically recognize single PG isozymes important for Botrytis virulence. To identify PGIPs that are able to efficiently inhibit BcPG1, we expressed it in the heterologous host Pichia pastoris, purified and tested its activity in the presence of PGIPs of Phaseolus vulgaris and Arabidopsis thaliana. We found that PvPGIP2 of P. vulgaris is a very efficient inhibitor of BcPG1 and that, when overexpressed in transgenic tobacco and Arabidopsis plants, it restricts B. cinerea colonization. We conclude that the ability of PvPGIP2 to limit fungal colonization in transgenic plants correlates with its inhibitory effect on BcPG1, and discuss the potential of PGIPs and the encoding genes as biotechnological tools to control the phytopathogenic fungus B. cinerea.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 67, Issue 2, August 2005, Pages 108-115
Journal: Physiological and Molecular Plant Pathology - Volume 67, Issue 2, August 2005, Pages 108-115
نویسندگان
Cinzia Manfredini, Francesca Sicilia, Simone Ferrari, Daniela Pontiggia, Gianni Salvi, Claudio Caprari, Matteo Lorito, Giulia De Lorenzo,