کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9147544 | 1165432 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of stretch-activated calcium permeable cation channels in freshly isolated myocytes of the cricket (Gryllus bimaculatus) lateral oviduct
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش حشره شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Stretch-activated channels (SACs) were investigated in myocytes isolated from the lateral oviduct in cricket Gryllus bimaculatus using the cell-attached or excised inside-out patch clamp technique. Application of both negative and positive pressure (10-100 cm H2O) into the patch pipettes induced the unitary channel current openings. The open probability (NPo) of the channel increased when negative pressure applied into the patch pipettes increased. The single channel conductance for this channel was approximately 20 pS with 140 mM Na+, K+, or Cs+ in the patch pipettes and was approximately 13 pS with 100 mM Ca2+ or Ba2+ in the patch pipettes. External application of Gd3+, La3+, Cd2+ and Zn2+inhibited the channel with the IC50 values of 14, 15, 28, and 18 μM respectively. Interestingly external application of TEA, a specific blocker of K+ channel, also inhibited this channel with IC50 value of 8.8 mM. These results show for the first time the presence of stretch activated Ca2+-permeable nonselective cation channel in myocytes isolated from the cricket lateral oviduct. The physiological significance of this channel in oviposition behavior is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Insect Physiology - Volume 51, Issue 5, May 2005, Pages 481-488
Journal: Journal of Insect Physiology - Volume 51, Issue 5, May 2005, Pages 481-488
نویسندگان
T. Numata, M. Yoshino,