کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9441734 | 1301567 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham](/preview/png/9441734.png)
چکیده انگلیسی
A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes, respectively. To ensure the detection of the pathogens in cooked ham, samples were enriched in both buffered peptone-water and Half Fraser broth. Subsequently, equal volumes of enrichment broths were mixed and DNA purification was performed prior to m-PCR reaction, saving considerable time and effort. The m-PCR also proved to be very useful as a simple and ready-to-go method for simultaneous confirmation of presumptive L. monocytogenes and Salmonella spp. colonies directly from agar plates without any DNA extraction steps.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 22, Issue 1, January 2005, Pages 109-115
Journal: Food Microbiology - Volume 22, Issue 1, January 2005, Pages 109-115
نویسندگان
A. Jofré, B. Martin, M. Garriga, M. Hugas, M. Pla, D. RodrıÌguez-Lázaro, T. Aymerich,