کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9472035 1321156 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stabilization of beneficial traits in Heterorhabditis bacteriophora through creation of inbred lines
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Stabilization of beneficial traits in Heterorhabditis bacteriophora through creation of inbred lines
چکیده انگلیسی
Serial culturing of organisms used for biological pest suppression often leads to detrimental genetic changes and loss of utility. We established that genetically homozygous inbred lines can deter beneficial trait decline in the entomopathogenic nematode, Heterorhabditis bacteriophora. Three inbred lines and the foundation population were serially cultured in the insect host, Galleria mellonella. Trait stability was evaluated by comparing serially cultured with non-cultured populations. Laboratory data indicated that serial culture of the foundation population (16 passages) resulted in more than a 30% loss in traits deemed beneficial for biological pest suppression i.e., virulence to an insect host (Diaprepes abbreviatus), reproductive capacity, heat tolerance (at 38 °C), and host-seeking ability. In contrast, the three inbred lines were impervious to decline in all beneficial traits. A greenhouse test targeting D. abbreviatus provided additional evidence that the biocontrol efficacy of the inbred lines remained stable during serial culture. Our results indicate that genetic factors played an important role in trait change, and creation of inbred lines may be a useful technique for maintaining beneficial traits.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biological Control - Volume 32, Issue 2, February 2005, Pages 220-227
نویسندگان
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