کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9478379 | 1324799 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Organ distribution and kinetics of Glutathione transferase from African river prawn, Macrobrachium vollenhovenii (Herklots)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
The distribution of glutathione transferase (GST) in the major organs of African river prawn (Macrobrachium vollenhovenii) was studied. All the organs studied had GST activity. The specific activity of the extract from the hepatopancreas was highest while that from the muscle lowest. Purified GST from the hepatopancreas which could conjugate glutathione (GSH) with only 1-chloro-2,4-dinitrobenzene (CDNB) and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBDCl) among some electrophilic substrates tested, had a KmNBDCl of 2.2 ± 0.12 mmol lâ1 while the KmCDNB was 2.03 ± 0.29 mmol lâ1. Chloride ion, a product of the enzymatic reaction readily inhibited the conjugation of CDNB with GSH with an I50 of 0.12 mmol lâ1, whereas chloride ion up to 0.6 mol lâ1 had no inhibitory effect on the conjugation of GSH with NBDCl. However, nitrite inhibited the two reactions but the Ki for the conjugation of NBDCl was lower than the Ki for the conjugation of CDNB. The enzyme had an optimum temperature of 40 °C and an activation energy of 35.1 kJ/mol. The overall results show that M. vollenhovenii GST (mvGST) uses different mechanisms for different electrophilic substrates. The high Km of mvGST for the electrophilic substrates may be a special physiological adaptation for effective xenobiotic detoxication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 71, Issue 2, 26 January 2005, Pages 193-202
Journal: Aquatic Toxicology - Volume 71, Issue 2, 26 January 2005, Pages 193-202
نویسندگان
Isaac Olusanjo Adewale, Adeyinka Afolayan,