کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9746788 | 1492409 | 2005 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultra-sensitive, high throughput and quantitative proteomics measurements
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ultra-sensitive, high throughput and quantitative proteomics measurements Ultra-sensitive, high throughput and quantitative proteomics measurements](/preview/png/9746788.png)
چکیده انگلیسی
We describe the broad basis and application of an approach for very high throughput, ultra-sensitive, and quantitative proteomic measurements based upon the use of ultra-high performance separations and mass spectrometry (MS). An overview of the accurate mass and time (AMT) tag approach and a description of the incorporated data analysis pipeline necessary for efficient proteomic studies are presented. Adjunct technologies, including stable-isotope labeling methodologies and improvements in the utilization of liquid chromatography (LC)-MS peak intensity information for quantitative purposes are also discussed. Related areas include the use of automated sample handling for improving analysis reproducibility, methods for using information from the separation for more confident peptide peak identification, and the utilization of smaller diameter capillary columns having lower volumetric flow rates to increase electrospray ionization efficiency and allow for more predictable and quantitative results. The developments are illustrated in the context of studies of complex biological systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Mass Spectrometry - Volume 240, Issue 3, 1 February 2005, Pages 195-212
Journal: International Journal of Mass Spectrometry - Volume 240, Issue 3, 1 February 2005, Pages 195-212
نویسندگان
Jon M. Jacobs, Matthew E. Monroe, Wei-Jun Qian, Yufeng Shen, Gordon A. Anderson, Richard D. Smith,