کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9899160 | 1543764 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Salmonella enterica SpvB-mediated ADP-ribosylation as an activator for host cell actin degradation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Salmonella enterica SpvB-mediated ADP-ribosylation as an activator for host cell actin degradation Salmonella enterica SpvB-mediated ADP-ribosylation as an activator for host cell actin degradation](/preview/png/9899160.png)
چکیده انگلیسی
The virulence-associated SpvB protein of Salmonella enterica is a mono (ADP-ribosyl)transferase defined to target mammalian actin. Exposure of Acanthamoeba rhysodes cell lysate with SpvB and [32P]nicotinamide adenine dinucleotide (NAD) was here observed to result in labeling of a protein of 43Â kDa that subsequently was identified as actin by immunoprecipitation. In parallel, ADP-ribosylation promoted degradation of the protozoan actin. SpvB-mediated actin degradation occurred in the presence of the serine protease inhibitor phenylmethylsulfonylfluoride (PMSF), but was inhibited upon addition of novobiocin, an inhibitor of mono (ADP-ribosyl)transferase activity, or upon addition of EDTA. Infection of A. rhysodes with SpvB-proficient S. enterica serovariant Dublin resulted in cytotoxicity and in characteristic SpvB-mediated actin degradation. Cells infected with SpvB-deficient bacteria showed a decrease in cytotoxicity and lack of actin degradation. Combining these results show that SpvB formally can ADP-ribosylate A. rhysodes actin but that the protozoan cell has the capacity to subsequently degrade ADP-ribosyl-tagged actin. These observations illustrate a hitherto undefined consequence of actin modification, and define a new pathway in the cellular actin dynamics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 295, Issue 4, 22 August 2005, Pages 201-212
Journal: International Journal of Medical Microbiology - Volume 295, Issue 4, 22 August 2005, Pages 201-212
نویسندگان
Dilek Tezcan-Merdol, Lars Engstrand, Mikael Rhen,