کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9955279 | 1568663 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ptychographic X-ray CT characterization of the osteocyte lacuno-canalicular network in a male rat's glucocorticoid induced osteoporosis model
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
Ptychographic X-ray computed tomography (PXCT) is a quantitative imaging modality that non-destructively maps the 3D electron density inside an object with tens of nanometers spatial resolution. This method provides unique access to the morphology and structure of the osteocyte lacuno-canalicular network (LCN) and nanoscale density of the tissue in the vicinity of an osteocyte lacuna. Herein, we applied PXCT to characterize the lacunae and LCN in a male Wistar rat model of glucocorticoid-induced osteoporosis (GIO). The ptychographic images revealed significant (pâ¯<â¯0.05) differences in the number of canaliculi originating from the lacuna per ellipsoidal surface unit, Ca.Nb (pâ¯=â¯0.0106), and the 3D morphology of the lacuna (pâ¯=â¯0.0064), between GIO and SHAM groups. Moreover, the mean canalicular diameter, Ca.Dm, was slightly statistically un-significantly smaller in GIO (152â¯Â±â¯6.5) nm than in SHAM group (165â¯Â±â¯8) nm (pâ¯=â¯0.053). Our findings indicate that PXCT can non-destructively provide detailed, nanoscale information on the 3D organization of the LCN in correlative studies of pathologies, such as osteoporosis, leading to improved diagnosis and therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone Reports - Volume 9, December 2018, Pages 122-131
Journal: Bone Reports - Volume 9, December 2018, Pages 122-131
نویسندگان
Antonia Ciani, Hechmi Toumi, Stéphane Pallu, Esther H.R. Tsai, Ana Diaz, Manuel Guizar-Sicairos, Mirko Holler, Eric Lespessailles, Cameron M. Kewish,