Keywords: (GlcNAc)n; N-acetylchitooligosaccharides; GlcNAc; N-acetyl-d-glucosamine; pNP-(GlcNAc)n; p-nitrophenyl N-acetylchitooligosaccharides; HPLC; high-performance liquid chromatography; RACE; rapid amplification of cDNA ends; Fish stomach chitinase; Substrate s
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NMR analysis of substrate binding to a two-domain chitinase: Comparison between soluble and insoluble chitins
Keywords: Cryptomeria japonica; A two-domain chitinase; Chitooligosaccharide; Chitin nanofibers; NMR; CBM18; carbohydrate-binding module family 18; CJP-4; a class IV chitinase from Japanese cedar pollen; CJP-4-Cat; a GH19 catalytic domain of CJP-4; CJP-4(E108Q) and
Crystal structure of a “loopless” GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center
Keywords: GH; glycosyl hydrolase; BcChi-A; an inverting GH19 chitinase from Bryum coronatum; BcChi-A-E61A; a mutant form of BcChi-A in which the catalytic acid Glu61 is substituted with alanine; GlcNAc; N-acetylglucosamine; (GlcNAc)n; β-1,4-linked oligosaccharides
Complete subsite mapping of a “loopful” GH19 chitinase from rye seeds based on its crystal structure
Keywords: GlcNAc; 2-acetamido-2-deoxy-β-d-glucopyranose; (GlcNAc)n; β-1,4-linked oligosaccharide of GlcNAc with a polymerization degree of n; RSC-c; wild type GH19 chitinase from rye seeds; RSC-c-E67Q/W72A; a double mutant RSC-c, in which Glu67 and Trp72 are muta
Chitinase-catalyzed hydrolysis of 4-nitrophenyl penta-N-acetyl-β-chitopentaoside as determined by real-time ESIMS: The 4-nitrophenyl moiety of the substrate interacts with the enzyme binding site
Keywords: HvChiII; family GH-19 class II chitinase from barley seeds, Horduem vulgare L.; GlcNAc; 2-acetamido-2-deoxy-d-glucopyranose; (GlcNAc)n; β-1,4-linked oligosaccharide of GlcNAc with a polymerization degree of n; (GlcNAc)5-pNP; 4-nitrophenyl glycoside of (G
A β-N-acetylhexosaminidase from Symbiobacterium thermophilum; gene cloning, overexpression, purification and characterization
Keywords: ORF; open reading frame; PCR; polymerase chain reaction; BAP; bacterial alkaline phosphatase; IPTG; isopropyl-β-d-thiogalactopyranoside; PBS; phosphate buffered saline; SDS; sodium dodecyl sulfate; PAGE; polyacrylamide-gel electrophoresis; LB; Luria-Be