
Bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-Δ1-dehydrogenase from Mycobacterium neoaurum JC-12
Keywords: AD, 4-androstene-3,17-dione; ADD, androst-1,4-diene-3,17-dione; DCPIP, 2,6-dichlorophenolindophenol; FAD, flavin adenine dinucleotide; HPLC, high-performance liquid chromatography; KSDD, 3-ketosteroid-Δ1-dehydrogenase; ksdd, the coding gene of 3-ketostero