High-level expression and characterization of the Bacillus subtilis subsp. subtilis str. BSP1 YwaD aminopeptidase in Pichia pastoris
Keywords: Amino acid mutation; Carbamide; Glycosylation; High activity; High yield; EDTA; ethylenediaminetetraacetic acid; PCR; polymerase chain reaction; SDS; sodium dodecyl sulfate; PMSF; phenylmethylsulfonyl fluoride; BSAP; aminopeptidase from Bacillus subtilis;