Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; E1, pyruvate dehydrogenase; E2, dihydrolipoyl acetyltransferase; E3, dihydrolipoyl dehydrogenase; E3BP, E3 binding protein; mt-, mitochondrial; MTS, mitochondrial targeting sequence; PDC, pyruvate dehydrogen
مقالات ISI BIFC، مکمل فلورسانس دو طرفه حرکتی (ترجمه نشده)
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Overexpression of MtWRKY76 increases both salt and drought tolerance in Medicago truncatula
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; ABA, abscisic acid; MYB, v-myb avian myeloblastosis viral oncogene homolog transcription factor; CBF, C-repeat binding factor; BIFC, bimolecular fluorescence complementation; DGE, digital gene expression profilingWRKY transcription factor; Salt stress; Dr
The untapped potential of tyrosine-based G protein signaling
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; cAMP, cyclic adenosine monophosphate; CDK5, cyclin-dependent kinase 5; CT, carboxyl terminus; FRET, Förster resonance energy transfer; GAP, GTPase-accelerating protein; GDI, Guanosine nucleotide dissociation
Interactions among SARS-CoV accessory proteins revealed by bimolecular fluorescence complementation assay
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; aa, amino acids; AD, activation domain; BD, binding domain; BiFC, bimolecular fluorescence complementation; Co-IP, co-immunoprecipitation; E, envelope; EYFP, enhanced yellow fluorescent protein; M, membrane; N, nucleocapsid; NLS, nuclear localization sign
The identification of novel PMADS3 interacting proteins indicates a role in post-transcriptional control
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; Ade, adenine; AGO10, argonaute 10; BiFC, bimolecular fluorescence complementation; BY-2, N. tabacum L. cv. Bright Yellow 2; CDD, Conserved Domain Database; eIF3f, translation initiation factor 3f; GO, Gene Ontology; His, histine; Leu, leucine; N., Nicotia
Overexpression of the bZIP transcription factor OsbZIP79 suppresses the production of diterpenoid phytoalexin in rice cells
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; bZIP, basic leucine zipper; CaMV, Cauliflower Mosaic Virus; CBB, Coomassie Brilliant Blue; FLUC, firefly luciferase; GAL4DBD, GAL4 DNA-binding domain; GST, glutathione S-transferase; LUC, luciferase; MBP, ma
KPC2 relocalizes HOXA2 to the cytoplasm and decreases its transcriptional activity
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; CHX, cycloheximide; GST, glutathione S-transferase; HD, homeodomain; HOX, homeobox; ISH, in situ hybridization; KPC2, Kip1 ubiquitination-promoting complex 2; LMB, leptomycin B; ORF, open reading frame; r, r
Oligomerization of the UapA Purine Transporter Is Critical for ER-Exit, Plasma Membrane Localization and Turnover
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; MW, molecular weight; MM, minimal media; DDM, dodecyl-β-d-maltosidetransport; membrane sorting; trafficking; bimolecular fluorescence; endocytosis
Molecular recognition in the interaction of chloroplast 2-Cys peroxiredoxin with NADPH-thioredoxin reductase C (NTRC) and thioredoxin x
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; ET, electron transfer; FAD, flavin-adenine-dinucleotide; ITC, isothermal titration calorimetry; KA and KD, equilibrium association and dissociation constants, respectively; NTR, NADPH-dependent thioredoxin r
In vivo interaction between the tobacco lectin and the core histone proteins
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; BY-2, Bright Yellow 2; FRET, fluorescence resonance energy rransferBiFC; FRET; Histone; Interaction; Localization; Nictaba
Rice serine/threonine kinase 1 is required for the stimulation of OsNug2 GTPase activity
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; cDNA, complementary DNA; GFP, green fluorescent protein; GST, glutathione sepharose transferase; GTP, guanosine-5′-triphosphate; GDP, guanosine-5′-diphosphate; OsNug2, Oryza sativa nuclear/nucleolar GTPase 2
RING-type ubiquitin ligase McCPN1 catalyzes UBC8-dependent protein ubiquitination and interacts with Argonaute 4 in halophyte ice plant
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; Argonaute 4; Copine; In vitro enzyme assay; Post-translational modification; RING-type ubiquitin ligase; Ubiquitin-conjugating enzymeAGO4, Argonaute 4; AtERF53, Ethylene Response Factor53; BiFC, bimolecular fluorescence complementation; CPN1, copine1; MMZ
AtObgC-AtRSH1 interaction may play a vital role in stress response signal transduction in Arabidopsis
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; Chloroplast; AtObgC; AtRSH1; ppGpp; Stress response; Arabidopsis thalianaACT, aspartate kinase-chorismate mutase-TyrA; At, Arabidopsis thaliana; BiFC, bimolecular fluorescence complementation; CTD, C-terminal domain; DS 3.0 software, discovery studio vers
Excisions of a defective transposable CACTA element (Tetu1) generate new alleles of a CYCLOIDEA-like gene of Helianthus annuus
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; bp, base pair(s); BiFC, Bimolecular Fluorescence Complementation; Chry2, Chrysanthemoides2; CMT3, CHROMOMETHYLASE3; CYC, CYCLOIDEA; DDM1, DNA METHYLATION1; EMSA, Electrophoretic Mobility Shift Assay; En/Spm, Enhancer/Suppressor–mutator; MET1, METHYLTRANSF
The enterohemorrhagic Escherichia coli effector protein NleF binds mammalian Tmp21
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; Effector; Enterohemorrhagic E. coli; NleF; Tmp21; Type III secretionA/E, attaching and effacing; BiFC, bimolecular fluorescence complementation; EHEC, enterohemorrhagic E. coli; EPEC, enteropathogenic E. coli; ER, endoplasmic reticulum; LEE, locus of ente
Cytochalasin D acts as an inhibitor of the actin–cofilin interaction
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; CBB, coomassie brilliant blue; CytoD, cytochalasin D; FDAP, fluorescence decay after photoactivation; FRAP, fluorescence recovery after photobleaching; YFP, yellow fluorescent proteinCytochalasin D; Cofilin;
ROPGEF1 and ROPGEF4 are functional regulators of ROP11 GTPase in ABA-mediated stomatal closure in Arabidopsis
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; GEF, guanine nucleotide exchange factors; CA, constitutively active; DN, dominant-negative; LUC, luciferase; LCI, luciferase complementation imaging; Y2H, yeast two-hybrid; BiFC, bimolecular fluorescence complementation; YFP, yellow fluorescence proteinRO
Arabidopsis heterotrimeric G protein β subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; ABA, abscisic acid; BiFC, bimolecular fluorescence complementation; GPCR, G protein-coupled receptor; GST, glutathione S-transferase; ORF, open reading frame; PLC, phospholipase C; PP2C, 2C-type protein phosphatase; RT-PCR, reverse transcription-PCR; SE,
Formation of P450 · P450 complexes and their effect on P450 function
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; 7-EFC, 7-ethoxy-4-trifluoromethylcoumarin; BiFC, bimolecular fluorescence complementation; CPR, NADPH–cytochrome P450 reductase; CYP, designation for specific cytochrome P450 enzymes; EPR, electron paramagnetic resonance; EROD, 7-ethoxyresorufin-O-deethyl
Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; 16OMT, 16-hydroxytabersonine 16-O-methyltransferase; BiFC, bimolecular fluorescence complementation; D4H, desacetoxyvindoline-4-hydroxylase; DAT, deacetylvindoline-4-O-acetyltransferase; TIA, terpenoid indole alkaloids; LCM, laser-capture microdissection;
OSBP-related protein 7 interacts with GATE-16 and negatively regulates GS28 protein stability
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; ER, endoplasmic reticulum; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; GS28, Golgi SNARE of 28 kDa; LC3, microtubule-associated protein 1 light chain 3; NSF, N-ethylmaleimide sensitive factor; 25-OH
Unraveling tobacco BY-2 protein complexes with BN PAGE/LC–MS/MS and clustering methods
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; ATP, adenosine-5′-triphosphate; BiFC, bimolecular fluorescence complementation; BN, blue native; BY-2, Nicotiana tabacum cv. Bright Yellow-2; COS1, coronatine insensitive1 suppressor; FDR, false discovery rate; GO, gene ontology; HRC, hierarchical cluster
Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: An in vivo and in vitro study
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; cp, chloroplast; SRP, signal recognition particle; BiFC, bimolecular fluorescence complementation; aa, amino acids; TS, transit sequenceSignal recognition particle; Chloroplast; Protein transport; Alb3; Translocase; Light-harvesting chlorophyll-binding pr
In vivo quantification of protein–protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; FRET, fluorescence resonance energy transfer; PCA, protein fragment complementation assay; VN, N-terminal fragment of Venus; VC, C-terminal fragment of Venus; FITC, fluorescein isothiocyanate; CV, coefficien
An MCP-Like Protein Interacts with the MamK Cytoskeleton and Is Involved in Magnetotaxis in Magnetospirillum magneticum AMB-1
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; MCP, methyl-accepting chemotaxis protein; MTB, magnetotactic bacteria; OATZ, oxic–anoxic transition zone; GFP, green fluorescent protein; BiFC, bimolecular fluorescence complementation; OPI, overproduction interference; MSP, magnetospectrophotometry; CCCP
Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; MbYTH, membrane-based yeast two hybrid; CESA, cellulose synthase; BiFC, bimolecular fluorescence complementation; TMD, transmembrane domain; TF, transcription factor; YFP, yellow fluorescent proteinSecondary cell wall; Protein–protein interaction; CESA; M
Protein complementation assays: Approaches for the in vivo analysis of protein interactions
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BIFC, bimolecular fluorescence complementation; PCA, protein-fragment complementation assay; GFP, green fluorescent protein; FP, fluorescent protein; DHFR, dihydrofolate reductase; PPI, protein–protein interaction; RET, resonance energy transfer; BIFC-RET
Bimolecular Fluorescence Complementation Analysis of Inducible Protein Interactions: Effects of Factors Affecting Protein Folding on Fluorescent Protein Fragment Association
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; GFP, green fluorescent protein; YFP, yellow fluorescent protein; CFP, cyan fluorescent protein; FKBP, FK506 binding protein; FRB, FKBP–rapamycin binding domainvisualization of protein interactions; bimolecul
The variety of complexes formed by EcR and Usp nuclear receptors in the nuclei of living cells
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; 20E, 20-hydroxyecdysone; BiFC, bimolecular fluorescence complementation; CFP, cyan fluorescence protein; EcR, product of EcR gene; FRAP, fluorescence recovery after photobleaching; LBD, ligand binding domain; NES, nuclear export signal; NLS, nuclear local
Characterization of the C-terminal ER membrane anchor of PTP1B
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; PTP, protein tyrosine phosphatase; TA proteins, tail-anchored proteins; TMD, transmembrane domainPTP1B; Tail-anchored protein; Transmembrane domain; BiFC; Bimolecular fluorescence complementation; ER membran
NOX5 variants are functionally active in endothelial cells
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; BrdU, 5-bromo-2′-deoxyuridine; BSA, bovine serum albumine; DCF, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate; DHE, dihydroethidium; DMEM, Dulbecco's modified Eagle medium; GFP, green flu
Subcellular Localization of Interacting Proteins by Bimolecular Fluorescence Complementation in Planta
Keywords: BIFC، مکمل فلورسانس دو طرفه حرکتی; BiFC, bimolecular fluorescence complementation; EYFP, YFP variant derived from EGFP; nEYFP, N-terminal part of EYFP; cEYFP, C-terminal part of EYFP; YFP, yellow spectral variant; nYFP, N-terminal YFP; cYFP, C-terminal YFP; GFP, green fluorescent protein;