Keywords: تیروئلاگلولین گاو; BUP; Buprenorphine; MAbs; Monoclonal antibodies; GC; Gas chromatography; LC; Liquid chromatography; MS; Mass spectrometry; BSA; Bovine serum albumin; BTG; Bovine thyroglobulin; ELISA; Enzyme-linked immunosorbent assay; PBS; Phosphate-buffered saline; TMB;