Keywords: سنتز پروتئین سلولی; CalB; Candida antarctica lipase B; CECF; continuous exchange cell-free; DsRed; Discosoma sp. red fluorescent protein; pNPP; p-nitrophenyl palmitate; sfGFP; superfolder green fluorescent protein; Cell-Free protein synthesis; Molecular chaperone; Protein fo
مقالات ISI سنتز پروتئین سلولی (ترجمه نشده)
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Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Metabolic pathway optimization; Genetic circuits; Metabolic engineering; Synthetic biology;
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Cancer therapeutics; Antimicrobial peptides/proteins; Antibodies; Membrane proteins; Non-standard amino acids;
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Biosensor; in vitro protein synthesis; Protein biosensor; TX-TL; CFPS;
Assessing translational efficiency by a reporter protein co-expressed in a cell-free synthesis system
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Translational efficiency; Co-expression; Assessment of gene expression;
Keywords: سنتز پروتئین سلولی; Cell-free synthetic biology; Cell-free protein synthesis; Protein engineering; Metabolic engineering; Artificial cell; Unnatural amino acids;
Keywords: سنتز پروتئین سلولی; Resistant bacteria; Environmental water; Cell-free protein synthesis; Paper-based biosensor; Paromomycin; Tetracycline; Chloramphenicol; Erythromycin; β-Galactosidase; Chlorophenol red-β-d-galactopyranoside;
Keywords: سنتز پروتئین سلولی; Amino acid-selective stable isotope labeling; Cell-free protein synthesis; Coding theory; Combinatorial selective labeling; Signal assignment;
Keywords: سنتز پروتئین سلولی; Noncanonical amino acid; Genetic code expansion; Cell-free protein synthesis; tRNA isoacceptor; Phosphoserine; Near cognate suppression;
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Unnatural amino acids; Protein engineering; Residue-specific incorporation
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Saccharomyces cerevisiae; Synthetic biology; In vitro translation; Cell-free biology; Protein expressionANOVA, analysis of variance; ATP, adenosine triphosphate; cAMP, cyclic adenosine monophosphate; CFPS, cell-free protein sy
Keywords: سنتز پروتئین سلولی; Cell-free biology; Cell-free protein synthesis; In vitro transcription and translation; Protein expression; Saccharomyces cerevisiae; Natural energy metabolism;
Keywords: سنتز پروتئین سلولی; Automation; Eukaryotic cell-free protein synthesis; Integral membrane protein; Non-canonical amino acid; Electrophysiology; Fluorescence modification; Amb210; amber stop codon at position corresponding to amino acid 210 in translated protein; AzF; p-Azido
Keywords: سنتز پروتئین سلولی; aatRNA; aminoacyl-tRNA; CECF; continuous-exchange cell-free; CFCF; continuous-flow cell-free; CFPS; cell-free protein synthesis; CuAAC; copper-catalyzed alkyne-azide cycloaddition; eRF1; eukaryotic release factor 1; IRES; internal ribosome entry site; nsA
Keywords: سنتز پروتئین سلولی; Ricin; Passive pumping; Cell-free protein synthesis; Luminescence; Toxin detection;
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; In vitro translation; Extract preparation; Heterologous expression; Noncanonical amino acids; Candida antarctica lipase B
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Microcompartmentalization; Semipermeable microcapsule; Microfluidics; Rupture-induced encapsulation
Keywords: سنتز پروتئین سلولی; Cell-free; Synthetic biology; Cell-free synthetic biology; Cell-free protein synthesis; Gene circuits;
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Transcription; Translation; High throughput
Keywords: سنتز پروتئین سلولی; CFPS, Cell-free protein synthesisResidue-specific incorporation; Unnatural amino acids; Streptavidin; Cell-free protein synthesis; Tryptophan
Keywords: سنتز پروتئین سلولی; E. coli; Escherichia coli; NMR; nuclear magnetic resonance; PEP; phosphoenolpyruvate; scFv; single chain Fv; BPTI; bovine pancreatic trypsin inhibitor; LYZ; lysozyme C; MWCO; molecular weight cut-off; GSH; reduced glutathione; GSSG; oxidized glutathione;
Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Streptomyces; In vitro transcription and translation; Synthetic biology; Protein expression;
Functional characterization of cell-free expressed Kv1.3 channel using a voltage-sensitive fluorescent dye
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Membrane protein; KV1.3 channel; Proteoliposome; Oxonol VI;
Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products
Keywords: سنتز پروتئین سلولی; Cell-free synthetic biology; Purified enzymes; Cell-free protein synthesis; Polyketides; Nonribosomal peptides; In vitro biosynthesis; Natural products;
Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression
Keywords: سنتز پروتئین سلولی; Hematopoietic cell kinase (HCK); X-ray crystallography; Cell-free protein synthesis; Solubility; Chaperone; Autophosphorylation; GFP; green fluorescent protein; CAT; chloramphenicol acetyl-transferase; HCK; hematopoietic cell kinase; TEV; tobacco etch vir
The E. coli S30 lysate proteome: A prototype for cell-free protein production
Keywords: سنتز پروتئین سلولی; GeLC-MS/MS; Pre-fractionation by 1D-SDS-PAGE coupled with liquid chromatography mass spectrometry; FDR; false discovery rate; ABC-transporters; ATP-binding cassette transporters; CECF; continuous exchange cell-free; CFPS; cell-free protein synthesis; em
Structure determination of supra-molecular assemblies by solid-state NMR: Practical considerations
Keywords: سنتز پروتئین سلولی; 1D, 2D, 3D, 4D; one-dimensional, two-dimensional, three-dimensional, four-dimensional; AM-XiX; Amplitude-Modulated XiX decoupling; AQ; acquisition time; BASE RFDR; BAnd SElective Radio Frequency Dipolar Recoupling; BMRB; Biological Magnetic Resonance Data
Direct translational analysis of electrophoretically separated DNA using a cell-free protein synthesis system
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Electrophoresis; Translational analysis; Screening;
N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae
Keywords: سنتز پروتئین سلولی; Difficult-to-express protein; Increase of protein expression; Escherichia coli; Saccharomyces cerevisiae; N-terminal peptide tag; Cell-free protein synthesis;
Bacterial cell-free expression technology to in vitro systems engineering and optimization
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Engineering; Optimization; Polysaccharide; ATP-regeneration; Machine-learning; Synthetic minimal cell;
Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks
Keywords: سنتز پروتئین سلولی; CFPS; Cell-free Protein System; Cell-free protein synthesis; Synthetic biology; Minimal cell; Biobricks; Micro compartments;
In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display
Keywords: سنتز پروتئین سلولی; Bispecific antibody; Cell-free protein synthesis; Display technology; Disulfide stabilization; PURE system; bsAb; bispecific antibody; scFv; single-chain Fv; taFv; tandem scFv; dsDb; disulfide-stabilized diabody; CDR; complementarity-determining region; F
Immobilization of proteins onto microbeads using a DNA binding tag for enzymatic assays
Keywords: سنتز پروتئین سلولی; scCro; OR consensus DNA; Transglutaminase 2; Manganese peroxidase; Flow cytometry; Cell-free protein synthesis
Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Membrane protein; Viroporin; Proteoliposomes; Hepatitis C virus;
A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro
Keywords: سنتز پروتئین سلولی; Selenoprotein; Selenocysteine; Non-canonical amino acid; Cell-free protein synthesis; In vitro translation
Enhanced functional expression of aquaporin Z via fusion of in situ cleavable leader peptides in Escherichia coli cell-free system
Keywords: سنتز پروتئین سلولی; Aquaporin Z; Cell-free protein synthesis; Leader peptide; Leader peptidase; Membrane protein
Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Site-directed fluorescence labeling; Translocation; Microsomal vesicles; Staudinger ligation; Eukaryotic
Synthesis of 2.3Â mg/ml of protein with an all Escherichia coli cell-free transcription-translation system
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Escherichia coli extract; Maltose; Synthetic biology; Long-lived cell-free transcription-translation; TX-TL; transcription-translation; iP; inorganic phosphate; 3-PGA; 3-phosphoglycerate; SDS-PAGE; sodium dodecyl sulfate
Pluripotency transcription factor Sox2 is strongly adsorbed by heparin but requires a protein transduction domain for cell internalization
Keywords: سنتز پروتئین سلولی; Sox2; Polyarginine; Transcription factor; Cell-free protein synthesis; Heparan sulfate proteoglycans;
Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair
Keywords: سنتز پروتئین سلولی; Non-natural amino acid; Cell-free protein synthesis; Escherichia coli; Aminoacyl-tRNA synthetase; Orthogonal tRNA; PANOx SP; Glu NMP;
Cell-free expression of a mammalian olfactory receptor and unidirectional insertion into small unilamellar vesicles (SUVs)
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Olfactory receptor OR5; Unidirectional membrane insertion; Proteolytic digestion assay; Rabbit reticulocyte lysate;
Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus
Keywords: سنتز پروتئین سلولی; Vibrio parahaemolyticus; Thermostable direct hemolysin (TDH); Cell-free protein synthesis; In vitro translation;
Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming
Keywords: سنتز پروتئین سلولی; CFPS, cell-free protein synthesis; NAA, non-proteinogenic amino acid; PTM, post-translational modification; acK, acetyllysine; meK, methyllysine; 4-nt codon, 4 nucleotide codonGenetic code reprogramming; Cell-free protein synthesis; Flexizyme; Methyllysin
Cell-free platforms for flexible expression and screening of enzymes
Keywords: سنتز پروتئین سلولی; Cell-free protein synthesis; Enzyme; Protein engineering; Metagenome; PCR; Isothermal amplification; High-throughput screening
Functional evaluation of candidate ice structuring proteins using cell-free expression systems
Keywords: سنتز پروتئین سلولی; Ice structuring protein; Antifreeze protein; Ice binding protein; Cell-free protein synthesis; In vitro translation
Production of meganucleases by cell-free protein synthesis for functional and structural studies
Keywords: سنتز پروتئین سلولی; Meganuclease; I-CreI; Cell-free protein synthesis; Protein solubility; NMR
Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis
Keywords: سنتز پروتئین سلولی; PET; positron emission tomography; EGFRvIII; epidermal growth factor receptor variant III; scFv; single chain Fv; l-[methyl-11C]methionine; [11C]Met; CT; computed tomography; [18F]FDG; 2-[18F]-fluoro-2-deoxy-d-glucose; MWCO; molecular weight cut-off; Cell
Functional expression of Bacillus subtilis xylanase A in an Escherichia coli derived cell-free protein synthesis system and subsequent expression improvement via DNA gel technique
Keywords: سنتز پروتئین سلولی; CFPS; cell-free protein synthesis; SPS(s); signal peptide sequence(s); X-DNA; X-shaped DNA; AP; alkaline phosphatase; GDH; glucose dehydrogenase; PGA; penicillin G acylase; LipA; lipase A; DNS; 3,5-dinitrosalisylic acid; GFP; green fluorescent protein; OR
NMR study of xenotropic murine leukemia virus-related virus protease in a complex with amprenavir
Keywords: سنتز پروتئین سلولی; XMRV; Protease; Cell-free protein synthesis; NMR;
Synthesis of [11C]interleukin 8 using a cell-free translation system and l-[11C]methionine
Keywords: سنتز پروتئین سلولی; Interleukin 8; Imaging; Positron; Carbon-11; Cell-free protein synthesis; Unnatural amino acid;