Keywords: مسیر مرگ سلولی; Apoptosis; Caspases; Cryoinjuries; Cryopreservation; Freezing-thawing; Necrosis; Cell death pathway; Sea urchin; CMFSS; CaCl2 and MgCl2-free salt solution; EG; ethylene glycol; hsp; heat shock proteins; LN; liquid nitrogen; Me2SO; dymethyl sulfoxide; MTT;