High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites
Keywords: AcF, p-acetylphenylalanine; AzF, p-azidophenylalanine; Bpa, l-4-benzoylphenylalanine; Bpy, l-bipyridylphenylalanine; CECF, continuous exchange cell-free; CF, cell-free; Cmr, chloramphenicol resistance; CNF, p-cyanophenylalanine; Hco, 7-hydroxy-coumarin-4-