
Domain-specific free thiol variant characterization of an IgG1 by reversed-phase high-performance liquid chromatography mass spectrometry
Keywords: Monoclonal antibody; Free thiol; N-tert-butylmaleimide (NtBM); Alkylation; Reversed-phase high performance liquid chromatography (RP-HPLC); CDR; complimentarity determining region; CH; constant domain of the heavy chain; CL; constant domain of the light c