Keywords: طراحی پروتئین جدید; REU; Rosetta energy units; PDB; Protein Data Bank; SEC; size-exclusion chromatography; GuHCl; guanidine hydrochloride; DSS; 4,4-dimethyl-4-silapentane-1-sulfonic acid; pl; isoelectric point; computational protein design; de novo protein design; Rosetta; p