Combination of a modified block PCR and endonuclease IV-based signal amplification system for ultra-sensitive detection of low-abundance point mutations
Keywords: PCR; polymerase chain reaction; dNTP; deoxyribonucleoside triphosphate; UDG; Uracil-DNA Glycosylase; Endo IV; endonuclease IV; λexo; lambda exonuclease; HRM; high resolution melting; LOD; limit of detection; AS-PCR; allele specific PCR; COLD-PCR; co-ampl