Keywords: خطا مستقل PCR; Bacillus cereus; Error-prone PCR; Glycine oxidase; Site-directed mutagenesis; Substrate affinity
مقالات ISI خطا مستقل PCR (ترجمه نشده)
مقالات زیر هنوز به فارسی ترجمه نشده اند.
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در صورتی که به ترجمه آماده هر یک از مقالات زیر نیاز داشته باشید، می توانید سفارش دهید تا مترجمان با تجربه این مجموعه در اسرع وقت آن را برای شما ترجمه نمایند.
Error-prone PCR mutation of Ls-EPSPS gene from Liriope spicata conferring to its enhanced glyphosate-resistance
Keywords: خطا مستقل PCR; Error-prone PCR; 3D structure; EPSPS; Plant-sourced; Crop transformation;
Targeted mutagenesis: A sniper-like diversity generator in microbial engineering
Keywords: خطا مستقل PCR; Mutations; Targeted mutagenesis; Evolution; Synthetic biology; non-GMO; non-genetically modified organism; error-prone PCR; error-prone polymerase chain reaction; MAGE; multiplex automated genome engineering; TaGTEAM; targeting glycosylase to embedded arr
Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR
Keywords: خطا مستقل PCR; Endoglucanase; Error-prone PCR; Congo red; 3D-Structural modelling
Improving the activity of heparinase I by the directed evolution, its enzymatic properties and optimal conditions for heparin degrading by recombinant cells
Keywords: خطا مستقل PCR; Directed evolution; Error-prone PCR; Heparinase I; Enzymatic properties; Heparin degradation; Recombinant cells
Obtaining a mutant of Bacillus amyloliquefaciens xylanase A with improved catalytic activity by directed evolution
Keywords: خطا مستقل PCR; Xylanase; Mutant; Error-prone PCR; Catalytic activity; Thermostability; Hydrolysis
Engineering of global regulator cAMP receptor protein (CRP) in Escherichia coli for improved lycopene production
Keywords: خطا مستقل PCR; Escherichia coli; cAMP receptor protein; Error-prone PCR; Transcriptional engineering; Lycopene;
Error-prone pcr-based mutagenesis strategy for rapidly generating high-yield influenza vaccine candidates
Keywords: خطا مستقل PCR; Influenza A virus; Vaccine; Vaccine production; High-yield strain; Error-prone PCR;
Improved production of α-ketoglutaric acid (α-KG) by a Bacillus subtilis whole-cell biocatalyst via engineering of l-amino acid deaminase and deletion of the α-KG utilization pathway
Keywords: خطا مستقل PCR; l-Amino acid deaminase; α-Ketoglutarate; Proteus mirabilis; Whole-cell transformation; Error-prone PCR; Site-saturation mutagenesis
Improving catalytic efficiency of endo-β-1, 4-xylanase from Geobacillus stearothermophilus by directed evolution and H179 saturation mutagenesis
Keywords: خطا مستقل PCR; Xylanase; Directed evolution; Error-prone PCR; Site-saturation mutagenesis; Catalytic efficiency;
A single mutation within a Ca2 + binding loop increases proteolytic activity, thermal stability, and surfactant stability
Keywords: خطا مستقل PCR; AAPL, N-glutaryl-l-Ala-l-Ala-l-Pro-l-Leu-p-nitroanilide; DTT, dithiothreitol; LAS, sodium linear alkylbenzene sulfate; 195C, Tyr195Cys mutantSubtilisin; Error-prone PCR; Proteolytic activity; Thermal stability; Surfactant stability; Calcium ion
A new method for random mutagenesis by error-prone polymerase chain reaction using heavy water
Keywords: خطا مستقل PCR; Error-prone PCR; Heavy water; Random mutagenesis;
Enhancing the enzymatic activity of the endochitinase by the directed evolution and its enzymatic property evaluation
Keywords: خطا مستقل PCR; Directed evolution; Error-prone PCR; Endochitinase; Enzymatic property;
Engineered NADH-dependent GRE2 from Saccharomyces cerevisiae by directed enzyme evolution enhances HMF reduction using additional cofactor NADPH
Keywords: خطا مستقل PCR; Aldehyde reductase activity; Error-prone PCR; Evolutionary engineering; Stress tolerance
Error-prone PCR of global transcription factor cyclic AMP receptor protein for enhanced organic solvent (toluene) tolerance
Keywords: خطا مستقل PCR; cAMP receptor protein; Error-prone PCR; Transcriptional engineering; Global transcription factor; Random mutagenesis; Organic solvent tolerance; Toluene tolerance;
Thermostability improvement of Orpinomyces sp. xylanase by directed evolution
Keywords: خطا مستقل PCR; Error-prone PCR; Xylanase; Thermostability; Orpinomyces
Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries
Keywords: خطا مستقل PCR; Affinity selection; Asymmetric PCR; Diversity; Error-prone PCR; FN3 monobody; Kunkel mutagenesis; Library construction; M13 bacteriophage; Mutation rate; Mutazyme II;
Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli
Keywords: خطا مستقل PCR; Directed evolution; Protein engineering; Error-prone PCR; Kunkel mutagenesis; Megaprimers;
Isolation of a thermostable variant of Lip2 lipase from Yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved
Keywords: خطا مستقل PCR; Lipase; Error-prone PCR; High-throughput screening; Thermostability; Aggregation; Disulphide interchange
Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile
Keywords: خطا مستقل PCR; Thermoanaerobacter tengcongensis; Directed evolution; Error-prone PCR; Endoglucanase; Molecular Dynamics
A convenient and robust method for construction of combinatorial and random mutant libraries
Keywords: خطا مستقل PCR; Mutant library; DNA shuffling; Error-prone PCR; Ligase-independent cloning
Improvement of alkaline lipase from Proteus vulgaris T6 by directed evolution
Keywords: خطا مستقل PCR; Proteus vulgaris lipase; Directed evolution; DNA shuffling; Error-prone PCR
Directed evolution of acyl-CoA:diacylglycerol acyltransferase: Development and characterization of Brassica napus DGAT1 mutagenized libraries
Keywords: خطا مستقل PCR; Error-prone PCR; High-throughput screening; Membrane protein; Nile red; TAG synthase; Triacylglycerol biosynthesis; Saccharomyces cerevisiaeDGAT, acyl-CoA:diacylglycerol acyltransferase; epPCR, error-prone PCR; HTS, high-throughput system; Kb, kilo base
Efficient screening for new amino acid dehydrogenase activity: Directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid
Keywords: خطا مستقل PCR; Directed evolution; Biocatalysis; Phenylalanine dehydrogenase; Error-prone PCR; High-throughput screening; Parallel over-expression
Enhanced activity of yqhD oxidoreductase in synthesis of 1,3-propanediol by error-prone PCR
Keywords: خطا مستقل PCR; yqhD oxidoreductase; Error-prone PCR; Catalytic efficiency; 1,3-Propanediol
Protein domain library generation by overlap extension (PDLGO): A tool for enzyme engineering
Keywords: خطا مستقل PCR; Directed evolution; Random mutagenesis; Error-prone PCR; EstE; Esterase; Whole-cell biocatalyst
Improved thermostability and the optimum temperature of Rhizopus arrhizus lipase by directed evolution
Keywords: خطا مستقل PCR; Rhizopus arrhizus; Lipase; Stability; Directed evolution; DNA shuffling; Error-prone PCR
Engineering a β-carotene ketolase for astaxanthin production
Keywords: خطا مستقل PCR; β-carotene ketolase; Astaxanthin; Adonixanthin; Zeaxanthin; Ribosomal binding site; Error-prone PCR
Directed evolution and characterization of Escherichia coli glucosamine synthase
Keywords: خطا مستقل PCR; Error-prone PCR; Escherichia coli; Glucosamine synthase; Product inhibition; Recombinant protein solubility; Fru-6-P; fructose-6-phosphate; GlcN; glucosamine; GlcNAc; N-acetylglucosamine; GlcN-6-P; glucosamine-6-phosphate; GlmS; glucosamine synthase;
Reducing mutational bias in random protein libraries
Keywords: خطا مستقل PCR; Random mutagenesis; Error-prone PCR; DNA shuffling; Mutational bias;
Directed evolution of copy number of a broad host range plasmid for metabolic engineering
Keywords: خطا مستقل PCR; Broad host range plasmid; Replication protein; Plasmid copy number; Carotenoids; Error-prone PCR;