Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria—Application to a single-chain Fv fragment
Keywords: EDTA, ethylenediaminetetraacetic acid; EGFR, epidermal growth factor receptor; Fab, antigen binding fragment; Fv, variable fragment; mPEG, maleimide-PEG; scFv, single-chain Fv; Mw, molecular weight; PEG, polyethylene glycol; SDS–PAGE, sodium dodecyl sulfa