Purification and characterization of five alkaline, thermotolerant, and maltotetraose-producing α-amylases from Bacillus halodurans MS-2-5, and production of recombinant enzymes in Escherichia coli
Keywords: Ap, ampicillin; Cm, chloramphenicol; LB, Luria-Bertani; G2, maltose; G3, maltotriose; G4, maltotetraose; G5, maltopentaose; NMR, nuclear magnetic resonance; ORF, open reading frame; PAGE, polyacrylamide gel electrophoresis; PCR, polymerase chain reaction;