دانلود مقالات ISI درباره GdmCl، guanidinium chloride + ترجمه فارسی

تست های آنزیمی سریع برای لیتریتوریولین و ال آرژینین بر اساس پلت فرم تشخیص پیرو فسفات

Rapid enzymatic assays for l-citrulline and l-arginine based on the platform of pyrophosphate detection

Keywords: GdmCl، guanidinium chloride; ADI, arginine deiminase; ASS, argininosuccinate synthetase; BSA, bovine serum albumin; CV, coefficient of variation; GdmCl, guanidinium chloride; HRP, horseradish peroxidase; NOS, nitric oxide synthase; PEP, phosphoenolpyruvate; POX, pyruvate oxidase; PPD

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2014 سفارش ترجمه

Evidence of non-coincidence of normalized sigmoidal curves of two different structural properties for two-state protein folding/unfolding

Keywords: GdmCl، guanidinium chloride; g-cyt-c, goat cytochrome-c; GdmCl, guanidinium chloride; DSC, differential scanning calorimetry; CD, circular dichroismProtein stability; Two-state denaturation; Non-coincidence of denaturation curves; Differential scanning calorimetry; Urea denaturation;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Deletion of extra C-terminal segment and its effect on the function and structure of artemin

Keywords: GdmCl، guanidinium chloride; ASA, accessible surface area; CA, carbonic anhydrase; GdmCl, guanidinium chloride; IPTG, isopropyl β-d-thiogalactopyranoside; LB, Luria–Bertani; MD, Molecular Dynamic; Ni–NTA, nickel–nitrilotriacetic acid; PCR, polymerase chain reaction; pNPAc, p-nitrophe

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2011 سفارش ترجمه

Copper(II)-Induced Secondary Structure Changes and Reduced Folding Stability of the Prion Protein

Keywords: GdmCl، guanidinium chloride; PrP, prion protein; GdmCl, guanidinium chloride; mPrP, mouse PrP; FTIR, Fourier transform infrared spectroscopyCu2+; urea; unfolding; PrP; circular dichroism

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2011 سفارش ترجمه

Expression and purification of soluble E-Syt2: Low protein stability impedes tag removal

Keywords: GdmCl، guanidinium chloride; CD, circular dichroism; E-Syts, extended synaptotagmin-like proteins; GdmCl, guanidinium chloride; MCTPs, multiple C2 domain and transmembrane region proteins; PKC, protein kinase C; SEC, size exclusion chromatography; TM, transmembrane domainE-Syts; C2 d

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Modulating the Mechanical Stability of Extracellular Matrix Protein Tenascin-C in a Controlled and Reversible Fashion

Keywords: GdmCl، guanidinium chloride; FnIII, fibronectin type III; TNfn3, third fibronectin type III domain; AFM, atomic force microscopy; SMD, steered molecular dynamics; biHis, bihistidine; FNfn10, 10th FnIII domain from fibronectin; GdmCl, guanidinium chloride; wt, wild type; EDTA, ethylen

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2009 سفارش ترجمه

The Folding Pathway of the Antibody VL Domain

Keywords: GdmCl، guanidinium chloride; CDR, complementarity-determining region; GdmCl, guanidinium chloride; PBS, phosphate-buffered saline; AFM, atomic force microscopy; PPIase, peptidyl prolyl cis–trans isomerase; β2m, β2-microglobulin; EDTA, ethylenediaminetetraacetic acidprotein folding; p

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2009 سفارش ترجمه

Mapping of solution components, pH changes, protein stability and the elimination of protein precipitation during freeze–thawing of fibroblast growth factor 20

Keywords: GdmCl، guanidinium chloride; FGF-20, fibroblast growth factor 20; BSA, bovine serum albumin; GdmCl, guanidinium chloride; CD, circular dichroism; HPLC, high performance liquid chromatography; HEPES, 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid; TFA, trifluoroacetic acid; WCAS,

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2009 سفارش ترجمه

Consequences of Domain Insertion on the Stability and Folding Mechanism of a Protein

Keywords: GdmCl، guanidinium chloride; Ttrs, midpoint of a thermal unfolding transition; GdmCl, guanidinium chloride; [GdmCl]m, midpoint of a GdmCl-induced unfolding transition; [urea]m, midpoint of a urea-induced unfolding transition; m, cooperativity parameter of a denaturant (D)-induced unf

دانلود رایگان متن کامل مقاله ISI 15 صفحه سال انتشار : 2009 سفارش ترجمه

Spectroscopic evidence for the existence of an obligate pre-fibrillar oligomer during glucagon fibrillation

Keywords: GdmCl، guanidinium chloride; Glucagon; Kinetic; Pre-fibrillar specie; Oligomer; ExcimerG, glucagon; PG, N-terminal pyrene modified glucagon; ThT, thioflavin T; DLS, dynamic light scattering; AFM, atomic force microscopy; RH, hydrodynamic radius; GdmCl, Guanidinium Chloride; FRET, För

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

How Do Chemical Denaturants Affect the Mechanical Folding and Unfolding of Proteins?

Keywords: GdmCl، guanidinium chloride; AFM, atomic force microscopy; GdmCl, guanidinium chloride; GB1, B1 immunoglobulin-binding domain of protein G from Streptococcus; PBS, phosphate-buffered salinesingle molecule atomic force microscopy; protein folding and unfolding; chemical denaturation;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2008 سفارش ترجمه

Methanocaldococcus jannaschii adenylosuccinate synthetase: Studies on temperature dependence of catalytic activity and structural stability

Keywords: GdmCl، guanidinium chloride; AdSS, adenylosuccinate synthetase; MjAdSS, Methanocaldococcus jannaschii adenylosuccinate synthetase; GdmCl, guanidinium chloride; TCA, trichloroacetic acid; DHB, 2,5-dihydroxybenzoic acid; sAMP, succinyl-AMP; 6P-IMP, 6 phosphoryl-inosine monophosphate; D

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2008 سفارش ترجمه

Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains

Keywords: GdmCl، guanidinium chloride; D, denatured state; GdmCl, guanidinium chloride; N, native state; PDB, Protein Data Bank; PSBD, peripheral subunit-binding domain; TS, transition state; WT POB*, POB L146A Y166Wultrafast folding; transition state; phi-value; barrier limited

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2008 سفارش ترجمه

Increased Folding Stability of TEM-1 β-Lactamase by In Vitro Selection

Keywords: GdmCl، guanidinium chloride; β-lactamase, TEM-1 β-lactamase from E. coli; G3P, gene-3-protein; GdmCl, guanidinium chloride; EDTA, ethylenediaminetetraacetic acid; TM, midpoint of a thermal unfolding transition; [urea]1/2, midpoint of a urea-induced unfolding transition; ΔGD, Gibbs fr

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2008 سفارش ترجمه

Intense Neutral Drifts Yield Robust and Evolvable Consensus Proteins

Keywords: GdmCl، guanidinium chloride; ORF, open reading frame; GdmCl, guanidinium chloride; dPTP, 6(2-deoxy-beta-D-ribofuranosyl)-3,4-dihydro-8H-pyrimido[4,5-c][1,2]-oxazin-7-one triphosphate; 8-oxo-dGTP, 8-oxo-2'-deoxyguanosine triphosphateprotein evolution; neutral drift; mutational robustn

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2008 سفارش ترجمه

Quantifying the Structural Requirements of the Folding Transition State of Protein A and Other Systems

Keywords: GdmCl، guanidinium chloride; Acp, acyl phosphatase; BdpA, B-domain of protein A; biHis, bihistidine; G–S Amber 94, Garcia and Sanbonmatsu's modified version of Amber 94; GdmCl, guanidinium chloride; HX, hydrogen exchange; RCO, relative contact order; TSE, transition-state ensemble; U

دانلود رایگان متن کامل مقاله ISI 20 صفحه سال انتشار : 2008 سفارش ترجمه

Fully Reduced Ribonuclease A Does not Expand at High Denaturant Concentration or Temperature

Keywords: GdmCl، guanidinium chloride; DTNB, 5,5′-dithio-bis(2-nitrobenzoic acid); HX, hydrogen exchange; CD, circular dichroism; FRET, fluorescence resonance energy transfer; GdmCl, guanidinium chloride; Io, scattering intensity at zero angle; Rg, radius of gyration; RNase A, ribonuclease A,

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2007 سفارش ترجمه

Testing the paradigm that the denaturing effect of urea on protein stability is offset by methylamines at the physiological concentration ratio of 2:1 (urea:methylamines)

Keywords: GdmCl، guanidinium chloride; lzm, hen egg white lysozyme; RNase-A, ribonuclease-A; α-LA, apo-α-lactalbumin; TMAO, trimethylamine N-oxide; Tm, midpoint of denaturation; ΔGD, denaturational Gibbs energy change; GdmCl, guanidinium chloride; ε, molar absorption coefficient; [θ]222, mean

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

Lactoperoxidase folding and catalysis relies on the stabilization of the α-helix rich core domain: A thermal unfolding study

Keywords: GdmCl، guanidinium chloride; LPO, Lactoperoxidase; MPO, Myeloperoxidase; EPO, Eosinophil peroxidase; ESR, Electron Spin Resonance; CD, Circular Dichroism; DSC, Differential Scanning Calorimetry; GdmCl, Guanidinium Chloride; MTSL, 1-oxyl-2,2,5,5,-tetramethyl-Δ3-pyrroline-3-methyl)-met

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Thermodynamic and kinetic characterization of the association of triosephosphate isomerase: The role of diffusion

Keywords: GdmCl، guanidinium chloride; TIM, Triosephosphate isomerase; yTIM, TIM from Saccharomyces cerevisiae; GdmCl, Guanidinium chloride; SCM, Spectral Centre of MassDimer; Diffusion; Glycerol; Protein stability; TIM barrel

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Influence of the Internal Disulfide Bridge on the Folding Pathway of the CL Antibody Domain

Keywords: GdmCl، guanidinium chloride; ANS, 8-anilino-1-naphtalene sulfonic acid; CL, constant domain of the antibody light chain; CD, circular dichroism; DJ, double jump; EDTA, ethylene diamine tetra-acetic acid; GdmCl, guanidinium chloride; Ig, immunoglobulin; MAK33, monoclonal antibody from

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2007 سفارش ترجمه

Design of Disulfide-linked Thioredoxin Dimers and Multimers Through Analysis of Crystal Contacts

Keywords: GdmCl، guanidinium chloride; MODIP, modeling of disulfide bridges in proteins; PDB, Protein Data Bank; ASA, accessible surface area; DSC, differential scanning calorimetry; GdmCl, guanidinium chloride; CGH-10, 10 mM citrate, 10 mM glycine and 10 mM Hepes; ESI-MS, electrospray ionizat

دانلود رایگان متن کامل مقاله ISI 15 صفحه سال انتشار : 2007 سفارش ترجمه

Shape of the Free Energy Barriers for Protein Folding Probed by Multiple Perturbation Analysis

Keywords: GdmCl، guanidinium chloride; protein folding; Hammond behavior; rate-equilibrium free energy relationships; transition state movementREFER, rate-equilibrium free energy relationship; ASA, accessible surface area; GdmCl, guanidinium chloride

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2006 سفارش ترجمه

The Dimerization Mechanism of LIS1 and its Implication for Proteins Containing the LisH Motif

Keywords: GdmCl، guanidinium chloride; homodimerization; protein structure; neuronal migration; lissencephaly; protein stabilityPAF-AH, PAF-acetylhydrolase; a.a., amino acid residue(s); GdmCl, guanidinium chloride; TFE, trifluoroethanol; HSQC, heteronuclear single quantum coherence

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2006 سفارش ترجمه

Single-molecule FRET Study of Denaturant Induced Unfolding of RNase H

Keywords: GdmCl، guanidinium chloride; fluorescence resonance energy transfer; single-molecule spectroscopy; guanidinium chloride; protein folding; RNase HFRET, fluorescence/Förster resonance energy transfer; GdmCl, guanidinium chloride; SAXS, small-angle X-ray scattering; SANS, small-angle ne

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2006 سفارش ترجمه

In Vitro Evolution of a Hyperstable Gβ1 Variant

Keywords: GdmCl، guanidinium chloride; Gβ1, β1 domain of the streptococcal protein G; G3P, gene-3-protein of phage fd; TM, midpoint of a thermal unfolding transition; GdmCl, guanidinium chloride; [GdmCl]m, midpoint of a GdmCl-induced unfolding transition; m, cooperativity parameter of a GdmCl-

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2006 سفارش ترجمه

Φ-Analysis of the Folding of the B Domain of Protein A Using Multiple Optical Probes

Keywords: GdmCl، guanidinium chloride; NOE, nuclear Overhauser enhancements; RDC, residual dipolar coupling; GdmCl, guanidinium chloride; [GdmCl]50, the concentration of GdmCl at the midpoint of the transition; T-jump, temperature jumpΦ-value; error analysis; nucleation-condensation; framework

دانلود رایگان متن کامل مقاله ISI 15 صفحه سال انتشار : 2006 سفارش ترجمه

Small Proteins Fold Through Transition States With Native-like Topologies

Keywords: GdmCl، guanidinium chloride; biHis, bi-histidine divalent metal ion binding site; ctAcP, common-type acyl phosphatase; GdmCl, guanidinium chloride; LRO, long-range order; RCO, relative contact distance; TSE, transition state ensemble; ψο2 pt, ψο-value obtained from denaturant chevron

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2006 سفارش ترجمه

High-resolution Structures of Escherichia coli cDsbD in Different Redox States: A Combined Crystallographic, Biochemical and Computational Study

Keywords: GdmCl، guanidinium chloride; high-resolution crystal structures; cDsbD; oxidative protein folding; redox properties; protein stabilityTCEP, Tris(2-carboxyethyl) phosphine; GdmCl, guanidinium chloride; IAM, iodoacetamide; EC-Trx, E. coli thioredoxin; H-Trx, human thioredoxin; MD, mole

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2006 سفارش ترجمه